育肥方式对呼伦贝尔羊及其与杜泊羊杂交1代羔羊消化道酶活性的影响  

Effects of Fattening Patterns on Enzyme Activities of Digestive Tract of Hulun Buir Lambs and F1 Hybrid Lambs of Hulun Buir and Dorper Sheep

作　　者：孙娟[1] 闫素梅[1] 张莹[1] 吴铁梅[1] 王新朋[1] 常晨城 郭晓宇[1] 

机构地区：[1]内蒙古农业大学动物科学学院,呼和浩特010018

出　　处：《动物营养学报》2015年第5期1577-1585,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家公益性行业(农业)科研专项经费(201003061)

摘　　要：本试验旨在研究育肥方式对呼伦贝尔羔羊及呼伦贝尔羊与杜泊羊杂交1代羔羊小肠及胰腺酶活性的影响。采用2×2完全随机试验设计,第1因素为育肥方式,分自然放牧和放牧补饲;第2因素为品种,分呼伦贝尔羔羊及呼伦贝尔羊与杜泊羊杂交1代羔羊(由呼伦贝尔母羊与杜泊公羊杂交而来)。选择体重相近的健康4月龄断奶呼伦贝尔羔羊和呼伦贝尔羊与杜泊羊杂交1代羔羊各60只,各分为2组,每组30只。育肥阶段分为育肥前期、育肥后期2个阶段,共60 d。放牧补饲组羔羊每只每天补饲精料在育肥前期为0.27 kg,育肥后期为0.53 kg。育肥试验结束时,分别从每组羔羊中随机选取5只羔羊进行屠宰,取小肠及胰腺组织测定酶活性。结果表明:与自然放牧相比,放牧补饲可显著提高羔羊十二指肠的胰蛋白酶、脂肪酶及糜蛋白酶活性,空肠、回肠与胰腺的淀粉酶、脂肪酶及胰蛋白酶活性(P<0.05)。与呼伦贝尔羔羊相比,呼伦贝尔羊与杜泊羊杂交1代羔羊的十二指肠淀粉酶、糜蛋白酶活性,空肠及回肠的脂肪酶和胰蛋白酶活性显著增加(P<0.05);十二指肠胰蛋白酶、胰腺脂肪酶活性的增加趋于显著(0.05≤P<0.10)。总之,与自然放牧相比,放牧补饲能够提高羔羊的小肠及胰腺的消化酶活性,呼伦贝尔羊与杜泊羊杂交1代羔羊的小肠及胰腺的消化酶活性高于呼伦贝尔羔羊。This trial was conducted to investigate the effects of fattening patterns on enzyme activities of small intestine and pancreas of Hulun Buir lambs and F1 Hybrid lambs of Hulun Buir sheep and Dorper sheep. A 2× 2 complete random design was used, factor 1 was fattening pattern, including natural pasture and natural pas- ture＋concentrate supplementation; factor 2 was species, including Hulun Buir lambs and F1 Hybrid lambs of Hulun Buir sheep （ ♀ ） and Dorper sheep （ ♂ ）. A total of 120 healthy lambs （60 of each specie） with close body weight were selected, and lambs of each specie were divided into 2 groups with 30 lambs per group. The trial lasted for 60 days consisted of an early fattening period and a late fattening period. Fattening method of natural pasture＋concentrate supplementation group was that supplemented 0.27 kg concentrate per lamb every- day in early fattening period, and 0.53 kg per lamb everyday in late fattening period. At the end of the trial, five lambs from each group were slaughtered, and small intestine and pancreas samples were collected to deter- mine enzyme activities. The results showed as follows： comparing with natural pasture, natural pasture＋con- centrate supplementation could significantly increase the activities of trypsin, lipase and chymotrypsin of duode- num, and those of amylase, lipase and trypsin of jejunum, ileum and pancreas （P〈0.05）. Comparing with Hulun Buir lambs, the activities of amylase and chymotrypsin of duodenum, and those of trypsin and lipase of jejunum and ileum of F1 Hybrid lambs of Hulun Buir sheep and Dorper sheep were significantly increased. Trypsin activity of duodenum and lipase activity of pancreas tended to be increased （0.05≤P〈0.10）. In con- clusion, comparing with natural pasture, natural pasture＋concentrate supplementation can increase enzyme ac- tivities of small intestine and pancreas, furthermore, enzyme activities of F1 Hybrid lambs of Hulun Buir sheep and Dorper sheep are higher than those of Hulun Buir lam

关 键 词：羔羊 育肥方式 小肠 胰腺 酶活性 

分 类 号：S826[农业科学—畜牧学]