RP-HPLC法测定细叶鸢尾中二氢黄酮的含量  

Determination of a flavanone in Iris tenuifolia by RP-HPLC

作　　者：杨阳[1] 刘少静[1] 杜强[1] 赵长琦[2] 

机构地区：[1]西安医学院药学院,陕西西安710021 [2]北京师范大学生命科学学院,北京100875

出　　处：《安徽医药》2015年第5期862-865,共4页Anhui Medical and Pharmaceutical Journal

基　　金：十二五"重大新药创制"科技重大专项(No2013ZX09103001-019-001)

摘　　要：目的：建立RP-HPLC法，测定细叶鸢尾中一种具有抗老年痴呆症活性的二氢黄酮：5，2＇，3＇-三羟基-6，7-亚甲二氧基二氢黄酮。方法采用Agilent 1260HC－C18（4．6 mm ×250 mm，5μm）色谱柱，流动相为甲醇—1％冰醋酸梯度洗脱，流速为1 mL ·min^－1，检测波长为295 nm，柱温为25℃。结果该二氢黄酮在37．6～188 mg·L^－1浓度范围内线性关系良好，相关系数为0．9994；回收率为101．26％（RSD＝1．17％）。测得该活性化合物在细叶鸢尾中的含量非常高，6批药材中的含量为57．70～59．35 mg·g^－1。结论该检测方法准确，适用于细叶鸢尾中5，2＇，3＇-三羟基-6，7-亚甲二氧基二氢黄酮的测定。Objective This paper established a RP-HPLC method for determination of a flavanone exhibiting potential anti Alzheimer＇s disease activity in Iris tenuifolia.Methods The separation was carried out with an Agilent 1260HC-C18 column （4.6 mm ×250 mm, 5 μm）by gradient elution using methanol-1% acetic acid as mobile phase at a flow rate of 1 mL·min-1 .The detection wavelength was 295 nm and the column temperature was maintained at 25℃.Results Good linear relationship was observed in the range of 37.6~188 mg·L^-1（R2 =0.999 4）.The recovery rate was 101.26 %（RSD =1.17 %）.And the content of this active compound in Iris tenuifolia was very high,which reached 57.70~59.35 mg·g^-1 Conclusions The method had the advantages of acuteness and preci-sion,which was suitable for determination of 5,2＇,3＇-trihydroxy-6,7-methylenedioxyflavanone in Iris tenuifolia.

关 键 词：细叶鸢尾 二氢黄酮 5  2' 3'-三羟基-6  7-亚甲二氧基二氢黄酮 老年痴呆症 RP-HPLC法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]