Kinetic study of all-or-none hemolysis induced by cationic amphiphilic polymethacrylates with antimicrobial activity  

作　　者：Kazuma Yasuhara Kenichi Kuroda 

机构地区：[1]Graduate School of Materials Science,Nara Institute of Science and Technology [2]Department of Biologic and Materials Sciences,University of Michigan School of Dentistry

出　　处：《Chinese Chemical Letters》2015年第4期479-484,共6页中国化学快报（英文版）

基　　金：supported by the Department of Biologic and Materials Sciences,University of Michigan School of Dentistry,NSF CAREER Award (No.DMR-0845592 to KK);JSPS KAKENHI,Grant-in-Aids for Challenging Exploratory Research (No.25650053);Young Scientists (Nos.24681028 and 22700494 to KY)

摘　　要：To gain an understanding of the toxicity of antimicrobial polymers to human cells, their hemolytic action was investigated using human red blood cells （RBCs）. We examined the hemolysis induced by cationic amphiphilic methacrylate random copolymers, which have amino ethyl sidechains as cationic units and either butyl or methyl methacpjlate as hydrophobic units. The polymer with 30 tool% butyl sidechains （B3o） displayed higher hemolytic toxicity than the polymer with 59 tool% methyl sidechains （Ms9）. B3o also induced faster release of hemoglobin from RBCs than lVlsg. A new theoretical model is proposed based on two consecutive steps to form active polymer species on the RBC membranes, which are associated to RBC lysis. This model takes the all-or-none release of hemoglobin by the rupture of RBCs into account, providing new insight into the polymer-induced hemolysis regarding how individual or collective ceils respond to the polymers.To gain an understanding of the toxicity of antimicrobial polymers to human cells, their hemolytic action was investigated using human red blood cells （RBCs）. We examined the hemolysis induced by cationic amphiphilic methacrylate random copolymers, which have amino ethyl sidechains as cationic units and either butyl or methyl methacpjlate as hydrophobic units. The polymer with 30 tool% butyl sidechains （B3o） displayed higher hemolytic toxicity than the polymer with 59 tool% methyl sidechains （Ms9）. B3o also induced faster release of hemoglobin from RBCs than lVlsg. A new theoretical model is proposed based on two consecutive steps to form active polymer species on the RBC membranes, which are associated to RBC lysis. This model takes the all-or-none release of hemoglobin by the rupture of RBCs into account, providing new insight into the polymer-induced hemolysis regarding how individual or collective ceils respond to the polymers.

关 键 词：HemolysisAntimicrobial polymerAmphiphilic polymethacrylateKinetic analysisMembrane disruption 

分 类 号：O631.3[理学—高分子化学]