硝酸镓对临床分离金黄色葡萄球菌生物膜的体外清除作用  

In vitro clearance effects of gallium nitrate on biofilms of clinically isolated Staphylococcus aureus

作　　者：吴浩昕[1] 李蓉[2] 葛新[2] 

机构地区：[1]武警后勤学院附属医院,天津300162 [2]武警后勤学院,天津300309

出　　处：《中国感染控制杂志》2015年第4期223-226,共4页Chinese Journal of Infection Control

基　　金：武警后勤学院科研基金面上项目(WHM201202)

摘　　要：目的研究新型抗菌剂硝酸镓对金黄色葡萄球菌生物膜的清除作用,探索抑制细菌生物膜的新方法。方法采用结晶紫染色法,对临床分离的14株金黄色葡萄球菌进行生物膜阳性菌株筛选,检测硝酸镓对生物膜阳性菌株的最低抑菌浓度(MIC)和对生物膜的清除作用。结果 14株金黄色葡萄球菌中,生物膜阳性菌株9株。硝酸镓对金黄色葡萄球菌ATCC25923、生物膜阴性菌株以及9株生物膜阳性菌株的MIC均为16μg/mL。硝酸镓对金黄色葡萄球菌早期生物膜的清除率为(86.53±0.96)%,高于对晚期生物膜的清除率[(62.54±1.53)%](t=35.699,P<0.001)。结论硝酸镓具有抑制金黄色葡萄球菌增殖,清除其生物膜的作用,有望成为防治金黄色葡萄球菌感染的新型药物。Objective To study the effect of new type antiseptic gallium nitrate on the clearance of Staphylococcus aureus （S .aureus）biofilm,and explore new methods for suppressing bacterial biofilm formation.Methods Biofilm positive strains were screened among 14 clinically isolated S .aureus strains by crystal violet staining method,mini-mal inhibitory concentration （MIC）of gallium nitrate for biofilm positive strains and effect of gallium nitrate on the clearance of biofilm were measured.Results Of 14 S .aureus isolates,9 were biofilm positive strains;gallium ni-trate MICs for S .aureus ATCC 25923,biofilm-negative strain,and 9 biofilm positive strains were all 16 μg/mL;the clearance rate of gallium nitrate for early biofilm of S .aureus was significantly higher than mature biofilm （[86.53±0.96]% vs [62.54±1 .53]%,t=35.699,P 〈0.001 ）.Conclusion Gallium nitrate can inhibit growth of S .aureus strains and clear biofilm,it can be applied in the prevention and control of S .aureus infection.

关 键 词：金黄色葡萄球菌 生物膜 硝酸镓 清除 

分 类 号：R378.11[医药卫生—病原生物学]