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作 者:秦梦[1] 谢晓亮[2] 温春秀[2] 田伟[2] 刘玲娣[2] 尹春梅[1]
机构地区:[1]吉林农业大学中药材学院,吉林长春130118 [2]河北省农林科学院经济作物研究所,河北石家庄050051
出 处:《广东农业科学》2015年第7期48-54,共7页Guangdong Agricultural Sciences
基 金:国家科技支撑计划项目(2011BAI07B05);河北省自然科学基金(C2014301009)
摘 要:采用蒽酮比色法测定大青叶中可溶性糖含量,考马斯亮蓝G-250法测定大青叶中可溶性蛋白含量,氮蓝四唑光还原法测定超氧化物歧化酶(SOD)的活性,愈创木酚法测定过氧化物酶(POD)的活性,采用L9(34)正交表设计田间试验,研究不同施肥配比对板蓝根生理生化指标及生长指标的影响。结果表明,处理A9为最佳施肥处理,即施肥比例为9∶1∶8,复合菌肥施用量为1 125 kg/hm2,N、P、K施用总量为450 kg/hm2。氮肥有利于提高板蓝根产量,化肥与有机肥合理配施更有利于板蓝根产量的提高。The content of soluble sugar was determined by anthrone colorimetric method,the soluble protein content by coomassie brilliant blue G-250 method,the superoxide dismutase(SOD)activity was determined by nitrogen blue four triazole photoreduction method,the POD activity was detemined by guaiacol method. L9(34)orthogonal design experiment in the field was used to,study the effects of formula fertilization on the physiological and biochemical indexes and growth indexes of Radix isatidis. Results shoued that treatment A9 was the best fertilization,the fertilization ratio was 9∶1∶8,compound fertilizer application amount was 1 125 kg/hm2,N,P,K application amount was 450 kg/hm2. Nitrogen fertilizer is beneficial to improve the yield of R. isatidis,organic manure and chemical fertilizer combination is more advantageous to improve the yield of R. isatidis.
关 键 词:板蓝根 配方施肥 生理生化指标 生长指标 有机肥
分 类 号:S567[农业科学—中草药栽培] S147.2[农业科学—作物学]
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