福建省狼尾草属种质资源RAPD鉴定分析  

RAPD analysis of genetic diversity of Penniseum germplasm resources in Fujian

作　　者：叶健军 林洁荣[1] 焦文静[1] 陈碧成 

机构地区：[1]福建农林大学动物科学学院/国家菌草工程技术研究中心,福建福州350000

出　　处：《广东农业科学》2015年第7期128-132,共5页Guangdong Agricultural Sciences

基　　金：国家菌草工程技术研究中心开放基金-(JCJJ14001)

摘　　要：采用RAPD标记分析了18份狼尾草属种质的遗传多样性及亲缘关系,结果表明：19条引物在18份材料中共扩增出207条条带,其中176条具有多态性,多态比率85％,各引物扩增条带数为6～15条不等,遗传距离的变幅为0.0819～1.011,平均为0.4713;遗传相似系数变幅为0.4907～0.9352,平均为0.6871.聚类结果表明：遗传距离以0.7为值大致可以分为4类：巨菌草、热研4号王草、桂草1号王草、桂牧1号杂交象草、紫象草聚为一类;杂交狼尾草和细茎象草分别单独聚为一类;剩余的品种聚为第四类.The genetic diversity and relationships of 18 different Penniseum Rich germplasms were analyzed using Random Amplified Polymorphic DNA（RAPD）markers in order to provide essential information for the development and utilization of Pennisetum germplasm resources. RAPD analysis demonstrated that 19 random primers produced 207 loci, among which 176 were polymorphic loci,the percentage of polymorphic loci was 85%,and each primer produced 6-15 belts. Genetic distance changed from 0.0819 to 1.011,averaged 0.4713;Genetic similarity changed from 0.4907 to 0.9352,averaged 0.6871. According to clustering analysis,we classified the 18 materials into four groups by 0.7,Giant Juncao,P. purpureum×P. americanurn. cv. Reyan No.4,P. purpureum×P. americanurn. cv. Guicao No.1,（P. americanum×P. purpureum）×P. durpureum cv.Guimu No.1,P. purpureum Schum cv. Purple clustered together into one group;P. americanum×P. purpureum and P. purpureum Schum cv. Xijing each formed its own category,while the rest formed one category.

关 键 词：狼尾草 遗传多样性 RAPD分析 

分 类 号：S544.3[农业科学—作物学]