过表达HOPX基因对鸡脂肪生长发育重要基因表达的影响  

Effect of HOPX overexpression on expression of the important developmental genes in chicken adipose

作　　者：王宁[1] 史洪岩[1] 程敏[1] 孙婴宁[1] 李辉[1] 

机构地区：[1]农业部鸡遗传育种重点实验室,东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《东北农业大学学报》2015年第5期44-50,共7页Journal of Northeast Agricultural University

基　　金：国家重点基础研究发展计划(973计划)(2009CB941604);国家肉鸡产业技术体系项目(CARS-42);黑龙江高校科技创新团队建设项目(2010td02)

摘　　要：为揭示HOPX基因在鸡脂肪生长发育中的作用,研究采用Real-time RT-PCR和启动子荧光素酶报告基因技术,分析过表达HOPX基因对鸡脂肪生长发育重要基因的表达及其启动子活性的影响。结果表明,与空载体对照组(p CMV-HA vector)相比,HOPX基因能够促进A-FABP和PPARγ基因表达及其启动子活性,抑制KLF 7基因表达及其启动子活性,但对FAS基因表达及其启动子活性没有影响。研究结果显示,过表达HOPX基因可能促进鸡前脂肪细胞分化。To explore the functions of HOPX in chicken adipose development, in the present study, the effects of HOPX overexpression on mRNA expression and promoter activity of the important developmental genes in chicken adipose tissue were tested using Real-time RT-PCR and luciferase reporter assays. The results showed that compared to empty vector control, HOPX overexpression increased the mRNA expression and promoter activities of A-FABP and PPARy genes, decreased the mRNA expression and promoter activity of KLF 7 gene, but HOPX overexpression had no effect on mRNA expression and promoter activity of FAS gene. The results of this study indicate that HOPX may promote chicken preadipocyte differentiation.

关 键 词：HOPX基因 鸡 脂肪细胞分化 发育 

分 类 号：S831[农业科学—畜牧学]