焦炉逸散物职业性接触致P53基因第5和第8外显子损伤分析  

Occupational exposure to the coke oven emissions(COEs)and it's genetic damage to the P53 exon 5 and 8

作　　者：漆骏[1] 李刚[1] 何越峰[2] 徐丹先[1] 宋家卫 凡兴卫 刘阳[1] 段佳丽[1] 唐艳红[1] 林佶[1] 

机构地区：[1]云南省疾病预防控制中心,云南昆明650022 [2]昆明医科大学公共卫生学院,云南昆明650042 [3]曲靖市疾病预防控制中心,云南曲靖655099 [4]沾益县第二人民医院,云南曲靖655338

出　　处：《工业卫生与职业病》2015年第3期193-196,共4页Industrial Health and Occupational Diseases

基　　金：云南省科技厅资助项目(2009CD127)

摘　　要：目的观察焦炉逸散物职业暴露人群尿液中1-羟基芘浓度水平与P53基因第5、第8外显子损伤的关系。方法选取某焦化厂作业工人239人作为研究对象,高效液相色谱法检测尿液1-羟基芘水平;实时荧光定量PCR扩增人群外周血淋巴细胞P53基因外显子5和外显子8,通过循环阈值推算损伤后扩增效率,间接计算损伤指数。结果炼焦车间和焦油车间作业人员尿液中1-羟基芘浓度高于备煤车间和甲醇车间作业人员尿液1-羟基芘浓度;尿中1-羟基芘校准浓度与P53基因第8外显子损伤指数存在正相关。结论焦炉逸散物职业性接触可致第8外显子损伤突变增加,而与第5外显子损伤突变关系不大。Objective To explore the relationship between urinary 1-hydroxypyrene （1-OHP） concentration of the coke oven workers and the genetic damage of the exon 5, exon 8 of the P53 gene. Methods 239 workers from a coke oven plant were recruited, high performance liquid detection（HPLC）were used to detect the urinary 1-OI-IP concentration, the exon 5 and exon 8 genetic damage index of the P53 gene were evaluate by the real-time PCR technique through relative threshold cycle method. Results The urinary 1- OHP concentration of workers in the cove-oven workshop and coke tar workshop were higher than those in the coal preparation workshop and methanol produce workshop~ adjusted urinary 1-OHP concentration were positive correlated with the P53 exon 8 damage index. Conclusions Coke-oven emissions exposure may increase the P53 exon 8 genetic damage, while with no relation with the exonS.

关 键 词：焦炉逸散物 职业暴露 P53基因损伤 

分 类 号：R135[医药卫生—劳动卫生]