工作场所空气中1,6-己二异氰酸酯的吸收液衍生-液相色谱检测方法  

Determination of Hexamethylene Diisocyanate in Workplace Air by Derivatization with Absorbing Liquid and Liquid Chromatography

作　　者：温忆敏[1] 沈朝烨[1] 梁卫玖 杨文慧 朱怡平[1] 林元杰 

机构地区：[1]上海市疾病预防控制中心化学品毒性检定所,上海200336 [2]长宁区疾病预防控制中心理化检验科,上海200051 [3]奉贤区疾病预防控制中心理化检验科,上海201400

出　　处：《环境与职业医学》2015年第5期481-485,共5页Journal of Environmental and Occupational Medicine

基　　金：2013年卫生标准制(修)订项目(编号:20130201)

摘　　要：[目的]建立工作场所空气中1,6-己二异氰酸酯（HDI）的吸收液衍生-液相色谱检测方法。[方法]样品采用冲击式采样管,色胺/二甲亚砜吸收液采样并衍生,高效液相色谱测定HDI衍生物浓度。[结果]该方法在0.050~10μg/m L范围内呈线性良好,回归方程？=6.9×106x-4.6×104,相关系数r=1.000。当采样体积为45 L时,HDI最低检出浓度为0.000 54 mg/m3,最低定量浓度为0.001 8 mg/m3。采样后的吸收液常温下可长期保存,14 d的下降率小于5%。工作场所空气中可能存在的其他异氰酸酯类化合物等,在该方法条件下对测定结果无干扰。[结论]该方法适用于工作场所空气中HDI的日常监测。[Objective] To establish a detection method for hexamethylene diisocyanate（HDI） in workplace air by derivatization with absorbing liquid and liquid chromatography. [Methods] Air samples were collected with impinger and absorbing liquid（tryptamine/dimethylsulfoxide） for derivatization. The HDI derivative concentration was detected by high performance liquid chromatography. [Results] The method showed a good linearity within the range of 0.050-10 μg/m L with the regression equation ？=6.9×106x-4.6×104 and the correlation coefficient r=1.000. The limit of detection and limit of quantitation of HDI were 0.000 54 mg/m3 and 0.001 8 mg/m3 respectively when the sampling volume was 45 L. The absorption liquid could be kept for long time under normal temperature after sampling, as the degradation rate was less than 5% after 14 days. Other derivatives of isocyanates in workplace air did not interfere with the determination. [Conclusion] The method is suitable for the routine monitoring of HDI in workplace air.

关 键 词：1 6-己二异氰酸酯 工作场所 空气 液相色谱法 吸收液采样 

分 类 号：R115[医药卫生—公共卫生与预防医学]