贵州地区结核分枝杆菌利福平耐药相关基因rpoB突变特征分析  

Analysis of characteristics of rpo B gene mutations associated with Mycobacterium tuberculosis resistance to rifampicin in Guizhou of China

作　　者：欧维正[1] 骆科文[1] 陈峥宏[2] 陈静[3] 王燕[1] 秦万[1] 蒙俊[1] 

机构地区：[1]贵阳市肺科医院检验科,贵阳550003 [2]贵阳医学院微生物学教研室,贵阳550002 [3]贵阳市肺科医院结核科,贵阳550003

出　　处：《中国临床药理学杂志》2015年第10期833-835,共3页The Chinese Journal of Clinical Pharmacology

基　　金：贵州省社会发展攻关基金资助项目(黔科合SY字[2013]3060号);贵阳市社会发展与民生科技基金资助项目(筑科合同[2013103]14号)

摘　　要：目的了解贵州地区结核分枝杆菌(MTB)对利福平耐药相关基因rpo B突变特征以便探讨适合本地区MTB对利福平耐药的快速筛选方法。方法用PCR-荧光探针法检测临床标本MTB核酸,阳性者(低浓度除外)用基因芯片检测rpo B基因,异常检测者再进行测序分析。结果 MTB核酸阳性患者3966例,阳性率22.72%(3966/17452)。基因芯片检测2738例中,552例有rpo B基因突变,突变率为20.16%(552/2738);9例异常检测者,经基因测序验证,分别为位点缺失、密码子插入、同密码子双碱基突变和多位点突变。rpo B基因突变类型有26种,以531,526,516,511,533,513和512位点突变为主,分别占56.23%,18.09%,9.67%,8.61%,4.57%,1.76%和0.18%。结论贵州地区MTB对利福平耐药相关基因rpo B突变特征与文献报道基本一致,基因芯片可作为本地区MTB利福平耐药的快速筛选方法。Objective To study characteristics of rpoB gene mutations associated with Mycobacterium tuberculosis（ MTB） resistance to rifampicin in Guizhou of China to discuss rapid screening methods of detecting MTB resistance to rifampicin.Methods MTB DNA of clinical samples were tested by fluorescent PCR .Low DNA concentration samples were excep-ted.All MTB DNA positive samples were tested by gene chip to screen rpoB gene mutation.The samples tested to be abnormal were further sequenced and aligned. Results MTB DNA of 3966 samples was positive.The positive rate was 22.72%（3966/17452 ）.All of the 2738 samples were tested by gene chip , of which 552 samples were detected to have gene mutations and the rate was 20.16%（ 552/2738 ） .Nine sam-ples of rpoB gene were tested abnormal .These 9 samples were confirmed to have nucleotide deletion , insertion, the same codon mutation of double base or multisite mutation .There were 26 types of gene mutations and mainly were at site 531 , site 526 , site 516 , site 511 , site 533 , site 513 and site 512 and the mutation rates were 56.23%, 18.09%, 9.67%, 8.61%, 4.57%, 1.76% and 0.18%, separately.Conclusion The characteristics of rpoB gene mutations associated with MTB resistance to rifampicin in Guizhou were basically the same as those in the literature reports.The gene chip can be used as the rapid screening method to＆amp;nbsp;detect MTB resistance to rifampicin in Guizhou .

关 键 词：结核分枝杆菌 利福平 RPOB基因 基因芯片 基因突变 

分 类 号：R978.3[医药卫生—药品] R968[医药卫生—药学]