孟鲁司特对肠缺血再灌注大鼠的肠黏膜细胞凋亡及相关蛋白表达的影响  

Effects of montelukast on intestinal ischemia-reperfusion injury and apoptosis pathway in rats

作　　者：吴深宝[1] 童秀萍[1] 洪小飞[1] 朱旭星[1] 应光荣[1] 周国雄[2] 沈卫东[3] 

机构地区：[1]温州医学院附属义乌中心医院消化科,浙江义乌322000 [2]南通大学附属医院消化科,江苏南通226019 [3]南通大学附属江阴医院消化科,江苏江阴214400

出　　处：《中国临床药理学杂志》2015年第10期843-846,共4页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省医学会临床科研基金资助项目(2012ZYC-A87);浙江省义乌市科技局基金资助项目(11-3-26)

摘　　要：目的探讨半胱氨酰白三烯受体1(Cys LTsR1)拮抗剂孟鲁司特对肠缺血再灌注(I/R)损伤的保护作用。方法大鼠肠I/R模型,大鼠随机分为假手术组、模型组、孟鲁司特(2 mg·kg-1体重)组,检测各组小肠含水量变化,末端脱氧核苷酸转移酶介导的d UTP缺口末端标记测定法(TUNEL)检测各组小肠黏膜细胞的凋亡,蛋白质印迹法检测各组Cys LTsR1、半胱氨酸天冬氨酸蛋白酶9(caspase 9)和半胱氨酸天冬氨酸蛋白酶8(caspase 8)的表达的变化。结果与模型组相比,孟鲁司特组小肠含水量、肠组织黏膜细胞凋亡、Cys LTR1、caspase 9和caspase 8的表达水平均明显降低(P<0.05)。结论孟鲁司特可以减少肠I/R肠黏膜细胞的凋亡,抑制caspase 8和caspase 9的表达可能是重要的凋亡机制。Objective To explore the role of cysteinyl leukotriene re-ceptor 1 （ cysteinyl leukotrienes receptor , CysLTsR1 ） antagonist monte-lukast protective effect on intestinal ischemia -reperfusion （ I /R） injury in rats.Methods Thirty rats were divided into 3 groups （ sham opera-tion group , model group and test group （ montelukast , 2 mg· kg -1 ） . The tissues near to terminal ileum in rats were extracted to detect the water weight.The apoptosis of intestinal mucosa was determined by ter-minal dexynucleotidyl transferase （ TdT ） -mediated Dutpnick end labe-ling（ TUNEL ） .The cysteinyl leukotrienes receptor 1, caspase 9 and caspase 8 were detected by Western blotting .Ressults Compared with model group , intestinal water content , intestinal mucosal cells apoptosis , CysLTR1,caspase 8 and caspase 9 expression levels of small intestine in test group were lower with statistical significance （ P 〈0.05 ） . Conclusion Montelukast reduces apoptosis of intestinal mucosal cells in rats after intestinal I /R, the mechanism may be related to the inhibition of expressions of caspase 8 and caspase 9.

关 键 词：孟鲁司特 肠缺血再灌注 细胞凋亡 半胱氨酸天冬氨酸蛋白酶9 半胱氨酸天冬氨酸蛋白酶8 

分 类 号：R965.1[医药卫生—药理学] R979.5[医药卫生—药学]