旱莲草水提取物对高同型半胱氨酸血症大鼠血脂及血管内膜的影响  

Effects of the aqueous extracts of Eclipta Prostrata L.on plasma lipid levels and vascular intima in rats with hyperhomocysteinemia

作　　者：黄川锋[1] 秦玖刚[1] 王爱梅[1] 刘荣志[1] 马瑜红[1] 刘庆春[1] 张冬[1] 

机构地区：[1]南阳医学高等专科学校基础医学部,河南南阳473003

出　　处：《中国临床药理学杂志》2015年第10期882-885,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河南省高等学校青年骨干教师计划基金资助项目(2011GGJS-285)

摘　　要：目的探讨旱莲草水提取物对高同型半胱氨酸血症(Hhcy)大鼠血脂及血管内膜的影响。方法通过喂饲高L-蛋氨酸饲料,复制HHcy大鼠模型。50只SD大鼠随机分为5组,正常组、模型组、洛伐他汀组(2 mg·kg-1)及旱莲草低、高2个剂量组(5,10 g·kg-1),每日灌胃给药。8周后,生化法测定血清同型半胱氨酸(Hcy)、总胆固醇(TC)、三酰甘油(TG)水平,酶联免疫吸附法检测一氧化氮(NO)、内皮素(ET)及血栓素B2(TXB2)水平,胸主动脉切片HE染色在光镜下观察血管形态学改变。结果与模型组相比,高剂量旱莲草组血清NO水平明显上升,其余各项指标均显著降低,胸主动脉血管形态基本正常。结论旱莲草水提取物能够有效降低Hhcy大鼠血清Hcy水平,具有调节血脂、保护血管内膜的作用。Objective To investigate the effects of the aqueous extracts of Eclipta Prostrata L.（ EPL） on plasma lipid levels and the structure of vascular intima in rats with hyperhomocysteinemia （ Hhcy ） . Methods High L -methionine diet was used to construct HHcy rat model.Fifty SD rats were randomly divided into five groups：normal group, model group, lovastatin group （2 mg· kg -1 ）, Eclipta Prostrata L.（EPL） low-and high-doses groups （5, 10 g· kg -1 ）.The drags were administrated by intragastric administration once a day . After 8 weeks administration , the level of serum homocysteine （ Hcy ） , total cholesterol （TC） and trigly-ceride （ TG） were detected by biochemical analysis.While nitric oxide （NO）, endothelin （ET） and thromboxane B2 （ TXB2 ） were measured by enzyme linked immunosorbent assay （ELISA）.The histopathological examination of the thoracic aortas were observed microscopically afterhematoxylin -eosin （ HE ） staining. Results Compared with model group , serum levels of Hcy , TG, TC, ET and TXB 2 were significantly decreased （ P〈0.05 ） in EPL groups and lovastatin group , while NO levels were significantly increased （ P〈0.05） in high dose EPL group.HE sections showed that the shape of thoracic aortas looks as normal .Conclusion The aqueous extract of EPL could effectively reduce serum Hcy and regulate plasma lipid levels ,thus it could alleviate vascular damage .

关 键 词：旱莲草 高同型半胱氨酸血症 血脂 血管内膜 

分 类 号：R285.5[医药卫生—中药学] R972.6[医药卫生—中医学]