液相色谱-串联质谱法测定饮用水中4种双酚类化合物  

Determination of four bisphenolic compounds in drinking water by liquid chromatography-tandem mass spectrometry

作　　者：胡小键[1] 张海婧[1] 王肖红[1] 丁昌明[1] 林少彬[1] 

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021

出　　处：《卫生研究》2015年第3期427-430,共4页Journal of Hygiene Research

基　　金：卫生行业科研专项经费(No.201002001)

摘　　要：目的建立同时测定饮用水中4种双酚类物质（双酚F、双酚A、四氯双酚A和四溴双酚A）的液相色谱-串联质谱的方法。方法 200 ml水样经C18固相萃取柱富集净化,甲醇洗脱后,用T3色谱柱（2.1 mm×150 mm,3μm）分离,在负电离模式下用串联质谱仪定性定量分析。结果 4种双酚类物质方法检出限为0.20-5.5ng/L,两个水平的加标回收率为87.1%-109.0%,日内精密度为6.3%-12.4%,日间精密度为4.5%-15.4%。应用该方法测定了15份水样,其中1份浸泡水中测出双酚A,含量为85.4 ng/L,其余样品中均未测出目标物。结论该方法可以测定水中4种双酚类物质,方法准确可靠,灵敏度高。Objective To simultaneously determine the four bisphenolic compounds （bisphenol F, bisphenol A, tetrachlorobisphenol A and tetrabromobisphenol A ） in drinking water by liquid chromatography tandem mass spectrometry. Methods 200 ml water sample was extracted by solid-phase extraction, eluted with methanol and analyzed by liquid chromatography tandem mass spectrometry under the MRM mode. The separation was carried out on a T3 column （2. 1 mm×150 mm,3μm ）. Results The limits of detection for the four bisphenolic compounds were in the range of 0. 20 - 5.5 ng/L. The mean recoveries at the two spiked levels were 87.1% -109.0% with the intra-day precision between 6.3% - 12.4% and inter-day precision between 4.5% - 15.4%. The method was applied for determination of 15 water samples. Conclusion The method was sensitive, precise and accurate.

关 键 词：双酚类化合物 固相萃取 液相色谱-串联质谱 饮用水 

分 类 号：R994.6[医药卫生—毒理学] R123.5[医药卫生—药学]