子午流注纳甲法对急性缺血性脑卒中大鼠神经元特异性烯醇化酶、S100B蛋白的影响  

Effects of Najia Method of Midday-midnight Point Selection for NSE and S100B Protein in Acute Ischemic Stroke Rats

作　　者：商俊芳[1] 姜华[1] 杨喜云[1] 张武德[1] 王金海[1] 李志东[1] 赵敏[1] 鲍英存[1] 黄春换[1] 

机构地区：[1]兰州大学第二医院,甘肃兰州730030

出　　处：《中国中医药信息杂志》2015年第6期54-57,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省中医药科学技术研究课题(GZK-2011-32)

摘　　要：目的观察子午流注纳甲法对急性缺血性脑卒中(AIS)模型大鼠血清神经元特异性烯醇化酶(NSE)、S100B蛋白含量的影响。方法选用SPF级SD雄性大鼠,采用大脑中动脉线栓法建立模型,按随机数字表法分为空白组、假手术组、模型组、循经组和纳甲组,空白组、假手术组和模型组不作治疗,循经组予循经辨证取穴针刺治疗,纳甲组予子午流注纳甲法针刺治疗,每日定时治疗1次,观察神经功能及脑梗死体积情况,检测血清NSE、S100B蛋白的含量。结果与模型组比较,循经组、纳甲组神经功能评分下降、梗死灶体积减小、梗死灶体积百分比降低(P<0.05,P<0.01),血清NSE、S100B蛋白含量降低(P<0.05,P<0.01),纳甲组效果总体优于循经组。结论子午流注纳甲法通过降低AIS模型大鼠血清NSE、S100B蛋白含量达到神经保护和治疗作用。Objective To observe the effects of Najia method of midday-midnight point selection for acute ischemic stroke （AIS） model rats onthe contents of NSE and S100B protein in serum. Methods SPF SD male rats were chosen to establish the models by middle cerebral artery bolt method. Rats were divided into blank group, sham-operation group, model group, channel-point group, and Najia method group by random number table method. Blank group, sham-operation group, and model group were in the absence of treatment, while the channel-point group received acupuncture treatment according to differentiation syndrome. Najia method group used Najia method of midday-midnight point selection to conduct acupuncture treatment once a day. Improvement of neural function and cerebral infarction volume were observed. The contents of NSE and S100B protein in serum were detected. Results Compared with model group, neurological function score, infarct volume and infarct volume percentage, and the contents of NSE and S100B protein in serum decreased in Najia method group and channel-point group （P〈0.05, P〈0.01）. The effects of Najia group were generally better than the channel-point group. Conclusion Najia method of midday-midnight point selection can decrease the content of NSE and S100B protein in serum of AIS model rats, so as to achieve the effects of neuroprotection and treatment.

关 键 词：子午流注纳甲法 急性缺血性脑卒中 S100B蛋白 神经元特异性烯醇化酶 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]