大黄素对重症急性胰腺炎大鼠舌组织水通道蛋白3表达的调节作用  

Regulating Effects of Emodin on the Expression of Aquaporin-3 in Tongue Tissues of Rats with Experimental Severe Acute Pancreatitis

作　　者：齐文杰[1] 张淑文[1] 王红[1] 张苗苗[1] 文艳[1] 

机构地区：[1]首都医科大学附属北京友谊医院感染暨急救医学科,北京100050

出　　处：《中国中医药信息杂志》2015年第6期69-72,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：北京市中医药科技发展基金项目(JJ2008-015)

摘　　要：目的探讨重症急性胰腺炎大鼠舌组织水通道蛋白3(AQP3)的表达及大黄素的调节作用。方法采用5%牛磺胆酸钠逆行胆胰管注入制作大鼠急性重症胰腺炎模型。将SD雄性大鼠随机分成假手术组、模型组、大黄素组,大黄素组于造模后每日予大黄素20 mg/kg灌胃,模型组及假手术组予等量生理盐水灌胃。造模后每日观察大鼠精神状态、厚腻苔出现情况及存活率;造模第5日后各组随机选取8只大鼠处死,留取标本,采用Western blot和实时定量PCR检测舌组织AQP3蛋白及基因表达。结果与模型组比较,大黄素组大鼠死亡率、厚腻苔发生率降低及厚腻苔发生明显减少,AQP3蛋白及基因表达明显降低(P<0.05)。至实验第5日,模型组存活11只大鼠,出现厚腻苔5只;与假手术组比较,模型组大鼠舌组织AQP3蛋白及基因表达均明显升高(P<0.05)。结论大黄素通过下调AQP3蛋白及基因表达,改善重症急性胰腺炎大鼠症状,减少厚腻苔的发生。Objective To investigate the expression of aquaporin-3 （AQP3） in tongue tissues of rats with experimental severe acute pancreatitis （SAP） and regulating effects of emodin. Methods Rat models with SAP were established by injecting 5% sodium taurocholate retrogradely into gallbladder and pancreas. SD male rats were randomly divided into sham-operation group, model group, and emodin group. After model establishment, rats in the emodin group received gavage with emodin 20 mg/kg each day, while rats in the model group and sham-operation group received gavage with normal saline. The mental state, thick greasy tongue fur and mortality of rats were observed every day after model establishment, and 5 days later, protein and genetic expression of AQP3 were detected by Western blot and RT-PCR. Results Compared with the model group, the mortality and the thick greasy tongue fur significantly decreased, the protein and genetic expression of AQP3 significantly decreased in the emodin group （P〈0.05）. On the 5th day, 11 rats in the model group survived, and 5 rats had thick greasy tongue fur. Compared with the sham-operation group, the protein and genetic expression of AQP3 in the model group were higher （P〈0.05）. Conclusion Emodin can improve the severity of SAP and decrease the incidence of thick greasy tongue fur significantly by reducing the protein and genetic expression of AQP3.

关 键 词：胰腺炎 大黄素 水通道蛋白3 基因表达 蛋白表达 大鼠 

分 类 号：R285.5[医药卫生—中药学]