半夏泻心汤及其拆方对胃电节律失常大鼠胃组织缝隙连接蛋白Cx43表达的影响  

Effects of Banxia Xiexin Decoction and Its Components on Protein Expression of Cx43 in Gap Junction in Gastric Tissue of Electrogastric Dysrhythmias Rats

作　　者：李翠[1] 张冬梅[2] 娄利霞[2] 赵明镜[2] 李丽娜[1] 陈萌[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京中医药大学中医内科学教育部重点实验室,北京100029

出　　处：《中国中医药信息杂志》2015年第6期75-79,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173192)

摘　　要：目的探讨半夏泻心汤调节胃运动的配伍规律。方法选取90只健康SD雄性大鼠,分为正常组10只和模型组80只。复制大鼠胃电节律失常模型,经胃电生理学指标评价后,根据半夏泻心汤的配伍特点,将其拆分为辛开、苦降、甘补、辛开苦降、辛开甘补、苦降甘补组,每组10只。各给药组按相同药物浓度不等体积连续灌胃4周。给药后进行胃电参数分析,采用免疫组化法、RT-PCR检测胃组织Cx43及其m RNA表达。结果与正常组比较,模型组Cx43及其m RNA表达升高;与模型组比较,各给药组胃电慢波频率变异系数降低,各给药组Cx43及其m RNA表达降低,其中辛开甘补组作用最为显著。结论半夏泻心汤调节胃运动机制可能与其降低缝隙连接蛋白Cx43及其m RNA表达有关,从而改变紊乱的胃电节律,达到改善胃运动功能的作用。Objective To discuss compatibility regularity of Banxia Xiexin Decoction adjusting gastric mobility. Methods Ninty healthy SD male rats were divided into normal group （10 rats） and model group （80 rats）. Rat models of electrogastric dysrhythmias were established. After the evaluation of gastric electrophysiology, Banxia Xiexin Decoction were divided into Xinkai, Kujiang, Ganbu, Xinkaikujiang, Xinkaiganbu, and Kujiangganbu groups according to the compatibility regularity of Banxia Xiexin Decoction, 10 rats for each group. Rats in each medication administration group received gavage with same concentration but different volume for 4 weeks. Each group was analyzed for gastric electrical parameters after administration. The expressions of Cx43 and Cx43 mRNA in the gastric tissue of rats in each group were analyzed by immunohistochemistry and real-time fluorescent quantitative PCR. Results Compare with the normal group, the expressions of Cx43 and Cx43 mRNA in the gastric tissue of rats increased significantly in the model group. Compared with the model group, variable coefficient of electrogastric slow wave frequency in all medication administration groups decreased obviously. Banxia Xiexin Decoction and its components had decreased the expression of Cx43 and Cx43 mRNA to some extent, among which Xinkaiganbu group had best effects. Conclusion The mechanism of Banxia Xiexin Decoction adjusting gastric motility maybe related to its function of decreasing the expressions of Cx43 and Cx43 mRNA, as to adjust disordered electrogastric rhythm.

关 键 词：半夏泻心汤 CX43蛋白 缝隙连接 胃运动 大鼠 

分 类 号：R285.5[医药卫生—中药学]