HPLC测定天麻成分对甲氧基苄醇在大鼠血液中的药代动力学参数  

Determination of Pharmacokinetic Parameters of P-methoxybenzyl Alcohol from Gastrodiae Rhizoma in Plasma of Rats by HPLC

作　　者：段小花[1] 王维丽[1] 吴霜[1] 颜汉文[1] 柳丽松 林青[1] 

机构地区：[1]云南中医学院,云南昆明650000

出　　处：《中国中医药信息杂志》2015年第6期99-102,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81160514);云南省教育厅科学研究基金项目(2013Y240)

摘　　要：目的采用高效液相色谱法（HPLC）测定天麻成分对甲氧基苄醇在大鼠血液中的药代动力学参数。方法采用灌胃和静脉注射2种方式给药,HPLC测定不同时间大鼠血液中天麻成分对甲氧基苄醇的浓度,DAS3.0软件计算药代动力学参数。结果大鼠血液中对甲氧基苄醇在0.63-321.17μg/mL浓度范围内线性关系良好,r^2=0.994 5;日内、日间精密度、绝对回收率和稳定性均在规定范围内。结论本方法操作简便,能较准确地测出天麻成分对甲氧基苄醇在大鼠血液中的药代动力学参数。Objective To determine the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats by HPLC. Methods Gavage and intravenous injection were employed for administration. HPLC was used to determine the concentrations of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats in different time points. The pharmacokinetic parameters were computed by DAS3.0. Results The linear range of P-methoxybenzyl alcohol in plasma was 0.63-321.17 μg/mL, r ^2=0.994 5. Intra-day accuracy, inter-day accuracy, absolute recovery and stability were in specified range. Conclusion The method is simple and accurate for the determination of the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats.

关 键 词：高效液相色谱法 对甲氧基苄醇 药代动力学 大鼠 

分 类 号：R284.1[医药卫生—中药学]