慢性乙型肝炎患者IL-1RN(T+8006C)位点基因多态性分析  

Analysis of gene polymorphism at the position IL-1RN(T+8006C) in patients with chronic hepatitis B

作　　者：万鸿君[1] 彭芳[1] 

机构地区：[1]武汉大学人民医院检验科,武汉430060

出　　处：《检验医学与临床》2015年第10期1389-1390,1392,共3页Laboratory Medicine and Clinic

摘　　要：目的探讨白细胞介素-1受体拮抗剂基因(IL-1RN)第2号外显子+8006T/C位点基因多态性在慢性乙型肝炎患者中的分布及其相关性。方法采用聚合酶链反应-限制性片段长度多态性的分析方法,对450例慢性乙型肝炎患者和420例对照者IL-1RN(T+8006C)位点基因多态性进行检测,计算并分析其等位基因和基因型频率。结果观察组和对照组IL-1RN(T+8006C)位点基因型的分布最常见为TT型,TC型次之,CC型则较为罕见;在不同年龄组、不同性别间其分布比较,差异无统计学意义(P>0.05);观察组C型等位基因及TC基因型频率明显低于正常对照组,差异有统计学意义(P<0.01)。结论 IL-1RN(T+8006C)位点等位基因及基因型的分布不存在年龄和性别间的区别,但与慢性乙型肝炎存在相关性,在乙型肝炎病毒感染中,C型等位基因可能具有保护作用。Objective To investigate the distribution and correlation of gene polymorphism in the second exon of interleukin-1 receptor antagonist gene at position ＋8006[IL-1RN（T＋8006C）]in patients with chronic hepatitis B.Methods The IL-1RN（T＋8006C）polymorphism was detected by polymerase chain reaction and restriction fragment length polymorphisms methods in 450 patients with chronic hepatitis B（observation group）and 420 healthy persons（control group）,then the genotype and allele frequencies were calculated and analyzed.Results In the observation group and control group,the TT genotype was the most frequent,then the TC genotype,and CC genotype was rare.The distribution of genotypes and allele was no different among different gender and age groups（P0.05）.The frequencies of TC genotype and C allele were lower in patients with chronic hepatitis B than that of the control group（P0.01）.Conclusion The distribution of IL-1RN（T＋8006C）genotypes and allele were no difference among different gender and age groups,but it has correlation with chronic hepatitis B.Allele C might be a protect factor against hepatitis B virus infection.

关 键 词：白细胞介素-1受体拮抗剂 基因 多态性 慢性乙型肝炎 

分 类 号：R512.62[医药卫生—内科学]