氡致BEAS-2B细胞早期恶性转化的研究  

Study of early stage malignant transformation of BEAS-2B cells upon radon exposure

作　　者：刘明星[1] 纪雅慧[1] 韦晔[1] 刘玉萍[1] 苏志刚[1] 黄欢欢[1] 李建祥[1] 

机构地区：[1]苏州大学公共卫生学院江苏省放射医学重点实验室,苏州215123

出　　处：《江苏预防医学》2015年第3期1-4,共4页Jiangsu Journal of Preventive Medicine

基　　金：国家自然科学基金资助项目(81172707);江苏省自然科学基金资助项目(BK2012618)

摘　　要：目的了解氡致BEAS-2B细胞早期恶性转化的特征。方法通过细胞气体染毒装置,将永生化的人支气管上皮细胞(BEAS-2B)暴露于放射性气体氡,通过测定经氡照射后细胞周期、凋亡率、线粒体膜电位,判断照射后BEAS-2B细胞是否存在早期恶性转化趋势。结果染氡后细胞G1期缩短,S期延长,染氡细胞传代后晚期代数细胞凋亡率相比早期代数细胞降低,线粒体膜电位降低。结论短期染氡后,细胞周期等指标发生变化,出现恶性转化趋势。Objective To study early stage malignant transformation characteristics of BEAS-2B cell upon radon exposure. Methods Immortalized human bronchial epithelial cell line （BEAS-2B）was exposed to radioactive gas radon by cell gas expo-sure device.Early stage malignant transformation trend of BEAS-2B cells was evaluated based on analysis of cell cycle,apopto-sis rate and mitochondrial membrane potential.Results Upon exposure of radon,G1 stage of BEAS-2B cells shortened,while S stage extended;apoptosis rate of later stage cells was lower than that of early stage cells after cell passage;mitochondrial membrane potential decreased.Conclusion Upon short exposure to radon,series changes were observed on indices such as cell cycle and etc,demonstrating malignant transformation trend.

关 键 词：BEAS-2B 细胞 氡 恶性转化 细胞周期 凋亡率 线粒体膜电位 

分 类 号：R33[医药卫生—人体生理学]