慢性乙型肝炎患者血清中miR-122和miR-22的表达水平与干扰素疗效的相关性研究  

Correlation of serum levels of miR-122 and miR-22 with therapeutic effect of interferon among patients with chronic hepatitis B

作　　者：姜跃炜[1] 叶瑾[1] 朱小区[2] 

机构地区：[1]温州市中医院检验科,浙江温州325000 [2]温州市中医院感染科

出　　处：《中国预防医学杂志》2015年第5期369-374,共6页Chinese Preventive Medicine

摘　　要：目的通过检测血清中的miR-122和miR-22,研究慢性乙型肝炎(慢乙肝)患者干扰素治疗前后血清中miR-122、miR-22水平的变化及意义。方法收集HBeAg阳性慢性乙型肝炎患者43例作为研究对象。以慢乙肝患者用干扰素治疗48周后是否发生e抗原血清学转换为界分为e抗原血清学转换组和e抗原血清学未转换组。检测两组血清miR-122、miR-22在干扰素治疗前后的表达水平。结果在干扰素治疗24周和48周后,两组中的血清miR-122表达水平差异均有统计学意义(P均<0.01);而在干扰素治疗48周时两组血清miRNA-22差异有统计学意义(P<0.01)。在慢乙肝患者血清中:血清miR-122与ALT、HBsAg定量、HBeAg定量之间有较好的线性关系(r值分别为0.562、0.637、0.752;P值均<0.01),血清miR-22和ALT之间无相关性(r=0.192,P=0.141),但与HBsAg定量、HBeAg定量之间有相关性(r值分别为0.291、0.345;P值分别为0.024、0.007)。e抗原血清学转换组中在干扰素治疗前后血清miR-122、miR-22的表达水平有下降,血清miR-122差异有统计学意义(P<0.01),血清miR-22差异无统计学意义(P=0.316)。而在e抗原血清学未转换组干扰素治疗前后的血清中miR-122和miR-22的表达水平变化不大,差异无统计学意义(P=0.819,P=0.971)。结论血清miR-122和miR-22有可能为干扰素治疗慢性乙肝患者过程中预测疗效有价值的指标。Objective To evaluate the correlation of serum levels of miR-122 and miR-22 with the therapeutic effect of interferon among patients with chronic hepatitis B（CHB）. Methods Forty-three CHB patients with positive HBeAg were enrolled into the study.They were divided into two groups based on the status of e antigen seroconversion after treatment with interferon for 48 weeks.Serum levels of miR-122 and miR-22 were determined before and after treatment with pegylated interferon. Results After 24 and 48weeks of interferon treatment,significant differences of miR-122 expression level were observed between patients in two groups（P〈0.01）.However,the difference of serum miR-22 level was significant only after pegylated interferon treatment for 48weeks（P〈0.01）.Strong linear correlations were observed between serum miR-122 level and ALT,HBsAg and HBeAg quantity（r= 0.562,0.637,0.752,respectively;P〈0.01）.Serum level of miR-22 was related to HBsAg and HBeAg quantity（r= 0.291,0.345;P = 0.024,0.007）,not to ALT（r=0.192,P=0.141）.In patients with e antigen seroconversion,serum levels of miR-122 and miR-22 decreased after interferon treatment with significant difference of miR-122（P〈0.01）.While,serum levels of miR-122 and miR-22 remained almost unchanged before and after the treatment in patients with no e antigen seroconversion（P=0.819,P=0.971）. Conclusions Serum levels of miR-122 and miR-22 may become valuable indicators for the evaluation of therapeutic effect of interferon among patients with CHB.

关 键 词：MIR-122 miR-22 慢性乙型肝炎 干扰素 

分 类 号：R512.62[医药卫生—内科学]