树突状细胞对慢性阻塞性肺疾病的诊断意义  

Diagnostic significance of dendritic cells for chronic obstructive pulmonary disease

作　　者：刘雪青[1] 姜鲁宁[2] 宋敏[1] 

机构地区：[1]济宁医学院,济宁272067 [2]济宁医学院附属医院,济宁272100

出　　处：《中国免疫学杂志》2015年第5期678-682,共5页Chinese Journal of Immunology

基　　金：济宁市科技局项目(2013jnwk54)

摘　　要：目的:检测慢性阻塞性肺疾病急性加重期(AECOPD)患者外周血树突状细胞(DCs)亚群的水平,探讨其与AECOPD的关系。方法:采用流式细胞术四色荧光分析法分别检测34例AECOPD患者及15例健康者的外周血树突状细胞亚群(mDCs和pDCs)的水平;采用肺功能仪对各组患者进行肺功能检查。结果:AECOPD组中GOLDⅠ级、GOLDⅡ级、GOLDⅢ-Ⅳ级各组的mDCs与pDCs的水平均明显高于对照组(P<0.05),且随着AECOPD患者肺功能严重程度的增加,mDCs与pDCs的水平也随之增加(P<0.05)。结论:AECOPD患者mDCs与pDCs的水平升高,且与病情的严重程度明显相关,提示DCs参与了慢阻肺的炎症反应过程。Objective ：To detect the levels of dendritic cells （DCs）in peripheral blood of patients with acute exacerbations of chronic obstructive pulmonary disease （AECOPD） and explore the relationship between the content of DCs with AECOPD. Methods： The levels of DCs subsets（ mDCs and pDCs）in peripheral blood with thirty-four cases of AECOPD and fifteen cases of healthy persons were measured by flow cytometry with four-color fluorescent analysis;and the pulmonary function tests in every subjects were evaluated by spirometry. Results： Compared with the control group, the levels of mDCs and pDCs inAE COPD groups with GOLD I , GOLD II , GOLD m and ClasslV were significantly higher（P〈0. 05 ）,and with the severity of lung function in AECOPD patients, the levels were also increased（ P〈0.05 ）. Conclusion： The levels of mDCs and pDCs inAE COPD patients were higher and significantly correlated with the severity of the disease, suggesting that DCs may be involved in the inflammation reaction process of COPD.

关 键 词：树突状细胞 慢性阻塞性肺疾病 流式细胞术 

分 类 号：R562.1[医药卫生—呼吸系统]