低温预处理和聚乙二醇处理对小麦花药出愈率的影响  

Effects of Low Temperature Pretreatment and PEG on Callus Induction Rate of Wheat Anther

作　　者：杨雪[1] 刘春雷[1] 王丁[1] 张丽琴[1] 王世杰[1] 常慧萍[1] 邢文会[1] 付瑞敏[1] 

机构地区：[1]河南教育学院生命科学系,河南郑州450046

出　　处：《河南农业科学》2015年第4期49-51,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31071413);河南省科技攻关重点项目(122102110189);河南省自然科学研究项目(2011B210002);河南省高等学校重点科研项目(15A210020)

摘　　要：以河南教育学院小麦育种研究中心培育的小麦新品系为供试材料,选用C17为基本培养基,分别研究了低温预处理1、2、3、4、5、6、7 d,添加0(CK)、80、100、120 mg/L的聚乙二醇(PEG)对小麦花药出愈率的影响。结果表明:低温预处理4 d的小麦花药出愈率最高(2.108%),且稳定性较好(变异系数0.067),与低温预处理2 d的小麦花药出愈率差异显著(P<0.05),与其他5个处理之间的差异极显著(P<0.01)。在培养基中加入80、100、120 mg/L PEG可显著提高小麦花药出愈率,尤其以100 mg/L PEG效果最佳。Taking the wheat lines bred by Henan Institute of Education as tested materials and C17 as the basic culture medium,the influence of different low temperature pretreatment times and different PEG concentrations added in the medium on wheat anther callus rate was studied. The results showed that the callus rate of wheat anther was the highest（2. 108% ） when it was pretreated by low temperature for 4 d, which had good stability（CV = 0. 067）. On the other hand,adding 80—120 mg / L of PEG in the medium could significantly increase the callus rate of wheat anther,the effect of 100 mg / L PEG being the best.

关 键 词：低温预处理 聚乙二醇 小麦花药 出愈率 

分 类 号：S512.1[农业科学—作物学]