10-羟基喜树碱及其衍生物对肝癌细胞HepG2抑制作用的研究  

Study inhibitory effect of 10-hydroxycamptothecin and its derivative by Hep G2 cells

作　　者：李梦颖[1,2] 余飞[1] 侯冰雪[2] 肖倩[2] 叶黎文 刘可[2] 李鹏[1] 陈琴华[1,2] 

机构地区：[1]湖北医药学院附属东风医院,湖北十堰442008 [2]湖北医药学院第二临床学院,湖北十堰442000

出　　处：《中医药导报》2015年第11期34-35,38,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家大学生创新训练项目(201310929003);湖北医药学院优秀中青年科技创新团队计划(2014CXG04)

摘　　要：目的：研究10－羟基喜树碱（10－HCPT）和7－乙基－10羟基喜树碱（SN－38）对人体肝癌细胞HepG2的抑制作用并进行比较。方法：体外培养的肝癌细胞HepG2细胞株为实验研究对象，将10－HCPT和SN－38分别设5个浓度组（100nM、200nM、400nM、600nM、800nM）；10－HCPT和SN－385个浓度组分别干预肝癌HepG2细胞24、48及72h后，MTT法检测10－HCPT和SN－38对肝癌细胞HepG2的抑制作用。结果：10－HCPT和SN－38在100～800nM浓度范围能抑制肝癌细胞14epG2增殖，抑制率呈浓度递增趋势，各组间两两比较差异有显著性（P〈0．05）；SN－38对肝癌细胞HepG2的抑制作用比10－HCPT更加明显，且存在着时间和剂量的依赖性（P〈0．05）。结论：10－HCPT和SN－38对肝癌细胞HepG2有比较明显的抑制作用，SN－38比10－HCPT对肝癌细胞HepG2抑制作用更强。Objective： To study and compare the inhibitory effect of 10-hydroxycamptothecin 10-hydroxycamptothecin （10- HCPT） and 7- ethyl-10- hydroxy camptothecin （SN-38） by human HepG2 cells. Methods： Cultured HepG2 cells in vivo were the experimental subjects. The 10-HCPT and SN-38 are located five concentrations （100nM, 200nM, 400riM, 600nM and 800nM）; After HepG2 cells were intervened in 24, 48 and 72h by five concentrations of 10-HCPT and SN-38, inhibitory effect on HepG2 cells of 10-HCPT and SN-38 were determined by MTT method. Results： 10-HCPT and SN-38 can inhibit HepG2 cell proliferation inhibition rate at 100-800nM concentration range and its proliferation inhibition rates were increased. The re- sults of was significant by statistical analysis（P〈0.05）; The inhibitory effect of SN-38 more pronounced than that of 10-HCP by hepatoma cell line HepG2, and there is a time and dose dependent manner（P〈0.05）. Conclusion： 10-HCPT and SN-38 has a relatively significantly inhibited for HepG2 hepatoma ceils, and the inhibition of HepG2 ceils of SN-38 was stronger than that of 10-HCPT.

关 键 词：10-羟基喜树碱 7-乙基-10羟基-喜树碱 MTT HEPG2 

分 类 号：R285.5[医药卫生—中药学]