脑脊液神经元性烯醇化酶生物参考区间的建立  

Establishment of biology reference interval of NSE in cerebrospinal fluid

作　　者：曹艳菲[1] 陈刚[2] 张丽娜[1] 董秀鹏[1] 寇筱囡[1] 崔颖[1] 曹秀华[1] 

机构地区：[1]大庆油田总医院检验科,黑龙江大庆163001 [2]大庆油田总医院神经外科,黑龙江大庆163001

出　　处：《国际检验医学杂志》2015年第10期1321-1322,1324,共3页International Journal of Laboratory Medicine

基　　金：黑龙江省卫生厅科研项目(2011-617)

摘　　要：目的：建立适合本实验室的脑脊液神经元特异性烯醇化酶（NSE）生物参考区间。方法参照美国临床实验室标准化委员会（NCCLS）C28‐A2文件推荐的方法，收集120例无神经系统疾病的个体建立脑脊液NSE生物参考区间，并参照中国合格评定国家认可委员会CL38：2012文件的要求对生物参考区间进行评审。结果建立的脑脊液NSE生物参考区间为0～3．14 ng／mL。脑脊液NSE浓度与年龄、性别无明显相关性（P＞0．05）。结论采用上述方法建立生物参考区间可靠、准确、科学、实用，符合临床要求，值得推广应用。Objective To establish the reference intervals of neuron specific enolase （NSE） in tumor markers in cerebrospinal fluid .Methods According to National Committee for Clinical Laboratory Standards document C 28‐A2 ,120 samples were collected to establish reference intervals .Then ,the established intervals were evaluated according to China National Accreditation Service for Conformity Assessment document CL38 ：2012 .Results The biological reference interval of NSE in cerebrospinal fluid was 0-3 .14 ng/mL .There was no significant correlation between cerebrospinal fluid NSE level and age ,sex （P〉0 .05） .Conclusion Method used in this study could be ensured reliable ,accurate ,scientific and practical ,desirable for clinical requirement and with great poten‐tial for clinical application .

关 键 词：生物参考区间 临床医学实验室 脑脊液 神经元特异性烯醇化酶 

分 类 号：R446.14[医药卫生—诊断学]