HBV感染者调节性T淋巴细胞与细胞因子研究  

Research of Treg and cytokines in patients with hepatitis B virus infection

作　　者：颜晓霞 陈锡莲[2] 李彩东[2] 吴斌[2] 段正军[2] 田鹏飞[2] 

机构地区：[1]兰州市肺科医院,甘肃兰州730046 [2]兰州市第二人民医院肝病研究所,甘肃兰州730046

出　　处：《国际检验医学杂志》2015年第10期1346-1348,共3页International Journal of Laboratory Medicine

基　　金：甘肃省兰州市科技局科技发展指导性计划项目(2013-ZD-06)

摘　　要：目的探讨乙型肝炎病毒(HBV)e抗原(HBeAg)阳性及阴性HBV感染者外周血CD4+CD25+调节性T淋巴细胞(Treg)、白细胞介素-18、γ-干扰素、转化生长因子β表达水平及临床意义。方法将175例HBV感染患者分为慢性乙型肝炎(CHB)组和慢性无症状HBV携带者(ASC)组,各组再分为HBeAg(+)组和HBeAg(-)组,检测外周血CD4+CD25+Treg、细胞因子及肝功能指标的水平。以健康者作为对照组。结果 HBeAg(+)与HBeAg(-)ASC组和CHB组CD4+CD25+Treg表达水平与对照组比较差异无统计学意义(P>0.05);HBeAg(+)与HBeAg(-)ASC组和CHB组细胞因子表达水平较对照组明显升高(P<0.05);HBeAg(+)ASC组外周血CD4+CD25+Treg表达水平与丙氨酸氨基转移酶、天冬氨酸氨基转基酶/血小板比值呈正相关(P<0.05)。结论 CD4+CD25+Treg和细胞因子在乙型肝炎慢性化过程中起重要作用;CD4+CD25+Treg表达水平与肝炎的炎性反应程度有一定关系。Objective To investigate the value and clinical significance of CD4＋CD25^＋ regulatory T cells （Treg） ,inteleukin‐18 , interferon‐γand transforming growth factor‐βin patients with hepatitis B virus （HBV） infection .Methods A total of 175 patients with HBV infection were divided into chronic type B hepatitis （CHB） group and chronic asympotomatic HBV carrier （ASC） group , which were further divided into hepatitis B e antigen （HBeAg） positive and negative groups .CD4^＋ CD25^＋ Treg ,cytokines levels and liver function were measured .Healthy subjects were enrolled into control group .Results Proportion of CD4^＋CD25^＋ Treg were without significant difference among healthy controls ,HBeAg（＋ ） and HBeAg（-） ASC groups ,and HBeAg（＋ ） and HBeAg（-） CHB groups （P〉0 .05） .Compared with control group ,cytokines levels were significant higer in CHB and ASC group （P〈0 .05） . CD4^＋CD25^＋ Treg level was significant positive correlation to alanine aminotransferase and ratio of aspartate aminotransferase to plateles in HBeAg（＋ ）ASC group （P〈0 .05） .Conclusion CD4＋CD25 ＋ Treg and related cytokines could play important roles in the course of CHB ,while CD4^＋CD25^＋ Treg expressing might be correlated with inflammatory degree of hepatitis .

关 键 词：乙型病毒性肝炎 调节性T淋巴细胞 细胞因子 乙肝E抗原 

分 类 号：R512.62[医药卫生—内科学]