南通地区2014年肠道病毒71型的分子流行病学研究  

Enterovirus 71 molecular epidemiology research in Nantong,2014

作　　者：张宏萍[1] 周敏[1] 陆仁飞[2] 李雪梅[2] 

机构地区：[1]南通市疾病预防控制中心,江苏南通226001 [2]江苏省南通市第三人民医院,江苏南通226001

出　　处：《国际检验医学杂志》2015年第10期1354-1355,1357,共3页International Journal of Laboratory Medicine

基　　金：南通市科技局资助项目(HS2013028)

摘　　要：目的：分析2014年江苏南通地区肠道病毒71型（EV71）的分子流行病学特征。方法采集52例手足口病患儿咽拭子标本，提取病毒核酸，采用实时荧光定量PCR（RT‐PCR）扩增病毒的VP1序列。获得的序列与来自其他地区的序列进行比对分析，构建系统发育树。结果52份临床标本中分离到12株 EV71 VP1序列，12株 VP1序列的核苷酸、氨基酸同源性为93．4％～99．1％，进化树分析显示12株VP1序列全部属于C4基因型的C4a亚型。结论2014年南通地区分离的EV71流行病毒株与近年来中国其他地区的流行株有相同的起源，均属于EV71 C4基因的C4a亚型。Objective To analyze enterovirus 71 （EV71） molecular epidemiology characteristics in Nantong region of Jiangsu , 2014 .Methods The pharyngeal swab samples were selected from 52 children with hand ,foot and mouth disease .After extracting the virus nucleic acid ,the EV71 VP1 gene of the virus were amplified by RT‐PCR amplification .The phylogenetic tree was con‐structed between isolated and EV71 reference strains from other parts of the country .Results 12 strains EV71 VP1 gene were iso‐lated from 52 cases of clinical specimens .The nucleotide and amino acid homology of 12 strains EV71 VP1 gene was 93 .4% -99 .1% ,the phylogenetic tree analysis showed that 12 strains of VP1 gene all belong to C4 genotype C4a subgenotypes .Conclusion The isolated strains in Nantong in 2014 are all the EV71 C4a subgenotypes of C4 genotype ,the same with the most of isolates in re‐cent years .

关 键 词：肠道病毒71型 基因特征 分子流行病学 

分 类 号：R725.1[医药卫生—儿科]