流式细胞仪性能评价方法的建立  

Establishment of evaluation methods for the performance of flow cytometer

作　　者：王小林[1] 李昂[1] 杨硕[1] 

机构地区：[1]北京大学第三医院检验科,北京100191

出　　处：《国际检验医学杂志》2015年第10期1366-1367,1369,共3页International Journal of Laboratory Medicine

摘　　要：目的建立流式细胞仪性能评价方法。方法基于《YY/T0588-2005流式细胞仪》行业标准,利用BriCyte E6流式细胞仪,建立适用于流式细胞仪性能评价的测试方案,包括荧光灵敏度、荧光线性、前向角散射光检测灵敏度、仪器分辨率、前向角散射光和侧向角散射光分辨率、倍体分析线性、携带污染率、表面标志物检测准确性、表面标志物检测重复性、仪器稳定性等。结果该流式细胞仪各项性能指标均满足行业标准规定的技术要求。结论上述性能指标测试方法可靠,能够对流式细胞仪性能进行全面评价。该方法对于流式细胞仪性能验证有一定的指导意义。Objective To Establish evaluation methods for the performance of flow cytometer .Methods Referring to the indus‐try standard YY/T0588‐2005 Flow Cytometry ,evaluating methods for the performance of BriCyte E6 flow cytometry was estab‐lished ,such as fluorescence sensitivity ,fluorescence linearity ,forward scatter sensitivity ,instrument resolution ,forward scatter/side scattering resolution ,DNA content linearity ,carry‐over rate ,accuracy of the cell surface marker ,reproducibility of the cell surface marker and instrument stability .Results The performance of BriCyte E6 met the requirements of industry standard .Conclusion The evaluation methods for the performance parameters could be reliable and could be used for the performance evaluation of flow cytometer .

关 键 词：流式细胞仪 性能评价 BriCyte E6 

分 类 号：R197.39[医药卫生—卫生事业管理]