PA-MSHA对脓毒症大鼠的肺组织炎症反应的影响  

Effects of Pseudomonas aeruginosa-mannose-sensitive hemagglutinin on the inflammatory response in lung tissues of septic rats

作　　者：凌美蓉 万健 邓星奇 孙克玉 刘梅 单红卫[1] 

机构地区：[1]第二军医大学长征医院急救科,上海200003 [2]上海市闵行区中心医院急诊科,上海201199

出　　处：《皖南医学院学报》2015年第3期214-218,共5页Journal of Wannan Medical College

基　　金：上海市医学重点专科建设计划项目(ZK2012A23)

摘　　要：目的:探讨铜绿假单胞菌甘露糖敏感血凝素(PA-MSHA)对脓毒症致急性肺损伤炎症反应的影响及可能机制。方法:健康SD大鼠120只按随机数字法分为假手术对照组(SC组,n=30)、脓毒症组(CLP组,n=30)、术前治疗组(Pre T组,n=30)和术后治疗组(Pos T组,n=30)。以盲肠结扎穿孔法(CLP)复制大鼠脓毒症模型。制模后12、24、36 h分别采集外周血测定血清细胞因子(TNF-α、IL-6、IL-10和TGF-β)并处死动物,实时RT-PCR方法检测各组肺组织Toll样受体4(TLR4)mRNA表达;计算肺损伤病理评分;采用流式细胞术检测大鼠外周血单核细胞TLR4阳性率。结果:脓毒症大鼠中Pre T组与CLP组和Pos T组相比,Pre T组血清细胞因子TNF-α、IL-6浓度降低而细胞因子IL-10、TGF-β浓度均高于CLP组和Pos T组(P<0.01)。脓毒症大鼠中Pre T组外周血单核细胞TLR4阳性率和肺组织TLR4 mRNA表达水平低于CLP组和Pos T组(P<0.05),肺组织病理学评分也优于另两组(P<0.01)。结论:术前给予PA-MSHA可以明显下调脓毒症大鼠的炎症反应,从而减轻肺病理损伤程度。Objective： To investigate the effects and the possible mechanism of Pseudomonas aeruginosa-mannose-sensitive hemagglutinin（ PA-MSHA） on the septic serum-induced inflammatory response in lung tissues of rats. Methods： 120 Sprague-Dawley（ SD） rats of healthy grade were randomized into sham operation group（ SC group,n = 30）,sepsis group（ CLP group,n = 30）,PA-MSHA treatment before CLP（ PreT group,n = 30）,and PA-MSHA treatment after CLP（ PosT group,n = 30） by random digit tables. Murine septic models were established through cecal ligation puncture（ CLP）. Rats in each group were sacrificed at 12,24 and 36 h after operation,and blood was collected to determine the serum TNF-α,IL-6,IL-10 and TGF-β concentration. Lung tissue samples were also obtained to determine the expression of TLR4 mRNA by real-time PCR. Pathological changes in lung tissues were observed with light microscopy after HE staining,and flow cytometry was used to examine the changes of TLR4 expression in peripheral blood monocytes.Results： Serum TNF-a and IL-6 concentration in PreT group were significantly lower,yet IL-10 and TGF-β concentration were significantly higher as compared with that of CLP and PosT groups（ P 〈0. 01）. Expression of TLR4 mRNA was markedly decreased（ P〈0. 05）,and the pulmonary pathological scores were better than the other two groups（ P〈 0. 01）. Conclusion： Preoperative administration of PA-MSHA can significantly reduce the inflammatory reaction and pathological injury of the lungs in septic rats.

关 键 词：脓毒症 急性肺损伤 PA-MSHA 细胞因子 TOLL样受体4 

分 类 号：R459.7[医药卫生—急诊医学]