部分景天属植物DNA条形码引物的筛选  

The Primers Selection of Sedum Plants DNA Barcoding

作　　者：张洋[1] 郝海叶 那冬晨[1] 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004

出　　处：《安徽农业科学》2015年第16期53-54,共2页Journal of Anhui Agricultural Sciences

基　　金：山西省大学生创新实验项目(SD2012CXSY-24)

摘　　要：[目的]对景天属植物分类所需用的相关DNA条形码引物进行筛选。[方法]以景天属(Sedum)中的佛甲草、八宝景天、华北景天、费菜、垂盆草5种植物为研究材料,采用CTAB法分步提取核DNA和叶绿体DNA,PCR扩增筛选引物,琼脂糖凝胶电泳检测PCR扩增产物。[结果]从psb A-trn H、rop B、rbc L、rpo C1、ITS 2、mat K 6个候选序列引物中初步筛选出适合景天属植物DNA条形码的4对引物——psb A-trn H、rop B、rpo C1、ITS 2,这4对引物的扩增率均达到100%。[结论]为景天属植物DNA条形码研究提供相关依据。[ Objective ] The aim was to select relevant DNA barcoding primers that the classification of Sedum plants needed. [ Method ] Choosing 5 species from Sedum plants as research materials, which was Sedum lineare Thunb, Sedum spectabile Boreau, Sedum tatarinowii Maxim,Sedumaizoon L. and Sedum sarmentosum Bunge. Chloroplasts DNA and nuclear DNA of 5 species from Sedum plants were extracted by CTAB method to step-by-step extracting, primers were selected by PCR, PCR productions were tested by agarose gelelectrephoresis. [ Result] Four pairs of primers（psbA-trnH,ropB,rpoC1 ,ITS 2） had been obtained preliminarily from six pairs candidate primers（psbA-trnH,ropB,rbcL, rpoC1 ,ITS 2,marK）. The amplification rate of four pairs primers reached 100%. [ Conclusion] The study provided related basis for studying DNA bareoding of Sedum plants.

关 键 词：景天属植物 引物筛选 DNA条形码 

分 类 号：S188[农业科学—农业基础科学]