高致病性PRRSV浓缩灭活疫苗的研制及免疫效果  

Development and Efficacy of Concentrated Inactivated Vaccines against Highly Pathogenic Porcine Reproductive and Respiratory Syndrome

作　　者：田永祥[1] 杨克礼[1] 段正赢[1] 周丹娜[1] 郭锐[1] 刘泽文[1] 袁芳艳[1] 孟丽[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所动物胚胎工程与分子育种湖北省重点实验室,湖北武汉430064

出　　处：《安徽农业科学》2015年第16期150-151,220,共3页Journal of Anhui Agricultural Sciences

基　　金：湖北省农业科技创新中心项目(2011-620-001-003);湖北省重点自然科学基金(2012FFA067);湖北省重点新产品新工艺研究开发项目(2012BBA15004);动物胚胎及分子育种湖北省重点实验室开放课题(2013ZD156)

摘　　要：[目的]研制高致病性PRRSV浓缩灭活疫苗。[方法]分别以病毒液、病毒浓缩液作为抗原,以甲醛、β-丙内酯为灭活剂,制成A、B、C、D 4种灭活疫苗,进行物理性状和无菌检验,安全检验合格后,与商品疫苗E进行免疫效果比较。[结果]4种疫苗均有良好的安全性。免疫后35 d,未浓缩抗原的A、C疫苗及商品疫苗E组抗体均未阳转,浓缩抗原的B、D疫苗组抗体阳转率分别达到80%和100%。[结论]浓缩病毒液制成的灭活疫苗比未浓缩病毒液制成的灭活疫苗及商品疫苗具有较好的免疫效果。[ Objective] The research aimed to develop vaccine against highly pathogenic porcine reproductive and respiratory syndrome. [ Method] Using virus and concentrated virus as antigen, and using formaldehyde and β-propiolactone as inactivator, four inactivated vaccines against highly pathogenic porcine reproductive and respiratory syndrome were prepared respectively. After the physical behavior, steriling test, and the safety test, the immunity effect of these four kieds of vaccines and one commercial vaccine were compared. [ Result] These four kinds of vaccine all had good safety. After immunization 5 weeks, the specific antibody titers produced by concentrated inactivated vaccines B and D were 80% and 100% respectively, while no specific antibody titers produced by the unconcentrated inactivated vaccines A, C and the commercial vaccine E. [ Conclusion ] The immune effect of inactivated vaccines prepared with concentrated virus was better than those prepared with unconcentrated virus and the commercial vaccine in this study.

关 键 词：高致病性PRRSV 浓缩 灭活疫苗 免疫效果 

分 类 号：S852.5[农业科学—基础兽医学]