壮腰通痹汤对大鼠腰椎间盘退变模型基质金属蛋白酶及组织形态学的影响  

Effects of Zhuangyao Tongbi Decoction on Matrix Metalloproteinases and Histomorphology of Lumbar Disc Degeneration of Rat Model

作　　者：潘泳鸿 陈少清[1] 林建平[1] 郑其开[2] 陈艺敏[2] 陈乐春[2] 苏友新[1] 

机构地区：[1]福建中医药大学,福建福州350122 [2]福建中医药大学附属康复医院,福建福州350003

出　　处：《按摩与康复医学》2015年第9期136-139,共4页Chinese Manipulation and Rehabilitation Medicine

基　　金：福建省自然科学基金项目,编号:2014J01346;福建省教育厅A类科技项目,编号:JA13163;福建省卫生厅青年科研课题,编号:2013-2-70

摘　　要：目的：观察壮腰通痹汤对大鼠椎间盘退变模型血清基质金属蛋白酶及椎间盘组织形态学的影响，探讨壮腰通痹汤治疗腰椎间盘退变性疾病的作用机理。方法：18只SD大鼠随机分成空白组、模型组及壮腰通痹汤组，采用纤维环穿刺法建立大鼠椎间盘退变模型，壮腰通痹汤灌胃治疗2周后用ELISA法测定各组血清MMP-1、MMP-3、TIMP-1的浓度，椎间盘组织行HE染色。结果：模型组MMP-1及MMP-3浓度均高于空白组（P〈0.05），壮腰通痹汤组MMP-1及MMP-3浓度均显著低于模型组（P〈0.05），壮腰通痹汤组TIMP-1浓度显著高于空白组及模型组（P〈0.05）；HE染色，模型组有明显病理改变，壮腰通痹汤组显示椎间盘组织病理损伤有一定修复。结论：壮腰通痹汤能提高椎间盘组织的自身修复能力，其椎间盘保护作用可能通过调节异常MMP-1、MMP-3、TIMP-1实现。Objective：To observe the effects of Zhuangyao Tongbi decoction on matrix metalloproteinases and histomorphology of lumbar disc degen-eration of rat model, and explore the mechanism of Zhuangyao Tongbi Decoction for degenerative lumbar disc disease. Methods：18 SD rats were randomly divided into blank group, model group and decoction group, establishing rat model of intervertebral disc degeneration with anulus punc-ture, after 2 weeks of gastric gavage of Zhuangyao Tongbi decoction, ELISA method was used to measure the serum concentration of MMP-1, MMP-3 and TIMP-1, intervertebral disc tissue with HE staining. Results：MMP-1 and MMP-3 concentrations of model group were higher than those of blank group （P〈0.05）, MMP-1 and MMP-3 concentration of decoction group were significantly lower than those of model group （P〈0.05）, and TIMP-1 concentration of decoction group was significantly higher than that of blank and model group （P〈0.05）; according to HE staining, model group had obvious pathological changes, decoction group showed that pathological injury of intervertebral disc tissue had certain repair capability. Conclusion：Zhuangyao Tongbi decoction can improve the self-repairing capability of intervertebral disc tissue, the protective effect of intervertebral disc is likely to be achieved according to regulating abnormal MMP-1, MMP-3 amd TIMP-1.

关 键 词：腰椎间盘退变性疾病 壮腰通痹汤 基质金属蛋白酶 组织形态学 

分 类 号：R681.53[医药卫生—骨科学]