Rho激酶抑制剂用于脑损伤治疗的初步实验研究  

The Preliminary Experimental Study of Rho Kinase Inhibitor Used for Brain Injury

作　　者：彭智涛 陈建良[1] 

机构地区：[1]广东省深圳市第四人民医院神经外科,广东深圳518000

出　　处：《吉林医学》2015年第11期2189-2191,共3页Jilin Medical Journal

摘　　要：目的:探讨Rho激酶抑制剂Y-27632对神经干细胞增殖分化的影响。方法:分离培养大鼠神经干细胞并进行鉴定,设置对照组、LPA组、Y27632组,连续培养2周,观察细胞单克隆个数、单克隆形成率、Ki67和GFAP的基因表达量及蛋白质表达水平。结果:分离培养的神经干细胞经免疫荧光检测Nestin、CD133均为阳性;软琼脂实验发现,LPA组单克隆个数及单克隆形成率较对照组及Y27632组显著升高(P<0.05),而Y27632组的单克隆个数及单克隆形成率与对照组相比差异无统计学意义(P>0.05);荧光定量及Western blotting结果显示,与对照组相比,LPA组GFAP基因表达量没明显变化(P>0.05),Ki67基因表达量升高(P<0.05),而Y27632组GFAP基因表达水平显著升高(P<0.05),而Ki67基因表达无变化(P>0.05)。结论:Rho激酶抑制剂Y27632可提高体外培养神经干细胞的GFAP表达水平,对于损伤神经细胞的修复具有重要作用。Objective To explore the effect of Rho kinase inhibitor Y -27632 on proliferation and differentiation of neural stem cells. Method The neural stem cells of the rats were isolated , cultured and identified. Blank control group, LPA treatment group and group Y27632 were set and cultured for two weeks. The number of monoelonal cells and the gene and protein expression level of Ki67 and GFAP were observed. Results The isolated and cultured neural stem cells were tested by immunofluorescence detection showing that Nestin and CD133 were positive ; It was found in soft agar assay that the monoclonal number of group LPA was the most while there was no significant difference between the group Y27632 and the control group （ P 〉 0. 05 ） ; The results of fluorescent quantitation and western blotting showed that compared with the control group, the expression level of GFAP in group LPA, did not change obviously （ P 〉 0. 05 ）, the expression level of Ki67 elevated while the gene expression level of group Y27632 increased significantly but the gene expression of Ki67 had no changes （P 〈 0. 05 ）. Conclusion Rho kinase inhibitor Y27632 can raise the expression level of GFAP of neural stem cells in vitro culture and plays an important role in the repair of the damaged nerve ceils.

关 键 词：神经干细胞 Y27632 干细胞分化 RHO激酶抑制剂 

分 类 号：R651.15[医药卫生—外科学]