消癌解毒方对肺癌A549细胞裸鼠移植瘤的抑制作用  

Inhibitory Effect of Xiao'ai Jiedu Recipe on Lung Cancer A549Cell Induced Xenograft Nude Mice Model

作　　者：朱际平[1] 沈健 晏婷婷[3] 

机构地区：[1]南京中医药大学附属医院呼吸科,江苏南京210029 [2]南京中医药大学附属医院药学部,江苏南京210029 [3]南京中医药大学,江苏南京210023

出　　处：《四川中医》2015年第5期41-43,共3页Journal of Sichuan of Traditional Chinese Medicine

基　　金：江苏省"青蓝工程"计划项目资助

摘　　要：目的:研究消癌解毒方对肺癌A549细胞裸鼠移植瘤的抑制作用并初步探讨其可能作用机制。方法:采用皮下接种肺癌A549细胞造成裸鼠移植瘤模型,待成瘤后将裸鼠随机分组并给予不同剂量的消癌解毒方,4周后肿瘤组织称重并测定裸鼠血清血管内皮生长因子(VEGF)含量。结果:给药组裸鼠肿瘤体积与模型对照组相比均增长较慢,4周后三组给药组裸鼠肿瘤重量均显著小于模型对照组(P<0.05,P<0.01),血清VEGF含量均显著低于模型对照组(P<0.01)。结论:消癌解毒方对肺癌裸鼠移植瘤模型肿瘤组织的生长有明显的抑制作用,通过降低血清VEGF含量可能是其作用机制。Objective： To study the inhibitory effect of Xiao＇ai Jiedu Recipe on the lung cancer A549cell induced xenografl nude mice model and its possible involved mechanism. Methods： The xenograft nude mice model was induced by inoculating sub- cutaneously lung cancer A549cell. Mice were randomly divided when xenograft tumors were established, and drugs were daily administered with different doses. Four weeks＇ experiment later, tumors were isolated and weighed, serum level of vascular endothelial growth factor （VEGF） were measured by ELISA. Results ： Tumor volume of mice in drugs teams grew slower than the controledteam. Four weeks later, tumor weight and serum level of VEGF of mice in drugs teams were significant low than the controlled team （P〈0. 05, P〈0. 01 ）. Conclusion： Xiao＇ai Jiedu Recipe can significantly inhibit the growth of lung cancer in xenograft nude mice model, reduction of serum level of VEGF is its possible involved mechanism.

关 键 词：消癌解毒方 肺癌 裸鼠移植瘤 抑制作用 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学]