迟钝爱德华氏菌耐药表型及4种耐药基因检测  

Detection of Drug Resistance and Four Drug Resistance Genes in Bacterial Pathogen Edwardsiella tarda

作　　者：葛慕湘[1] 靳晓敏[1] 张艳英[1] 房海[1] 陈翠珍[1] 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《水产科学》2015年第5期300-304,共5页Fisheries Science

基　　金：河北省重点基础研究项目(11960503D);现代农业产业技术体系河北省创新团队建设项目

摘　　要：从7起牙鲆迟钝爱德华氏菌感染病例中分离到130株致病菌,每个病例挑选5株共计35株,用K-B纸片扩散法检测其耐药表型;用PCR方法检测!-内酰胺类TEM基因、氨基糖苷类ant（3＂）-Ⅰ基因、磺胺类Sul3基因和四环素类tet（A）基因,分析耐药基因与耐药表型间的关系,以查明牙鲆源致病性迟钝爱德华氏菌不同类型常见耐药基因的携带状况,探讨耐药基因与耐药表型之间的相关性。结果显示,所检的35株迟钝爱德华氏菌均携带TEM基因和ant（3＂）-Ⅰ基因,62.9%（22/35）携带Sul3基因,未检出tet（A）基因;所有菌株均具有多重耐药性,大部分菌株耐抗菌类药物达7~9种;耐药基因的携带与耐药表型间存在较密切的相关性,但并非完全对应。The drug-resistance was tested by Kirby-Bauer method,and four drug-resistant genes including TEM,ant（3 ＂）-Ⅰ,Sul3 and tet（A）genes againstβ-lactamase genotypes,aminoglycoside,sulfonamides and tetracyclines were detected in 35strains（five strains each case）selected from 130 strains isolated from seven cases of Japanese flounder Paralichthys olivaceus infected by Edwardsiella tarda by PCR method to investigate the correlation between the drug resistance phenotypes and the drug resistance genotypes in the bacterial pathogen.The results showed that all detected strains had TEM and ant（3＂）-Ⅰgenes,62.9%（22/35）of the strains carrying Sul3 gene,and no tet（A）gene was detected in the experiment.All strains were found to be multi-drug resistant,most of which were resistant to 7—9antibiotics.There was a close relationship between the drug-resistance phenotype and the related resistance genes,without entirely corresponding.

关 键 词：迟钝爱德华氏菌 耐药表型 耐药基因 

分 类 号：S917.1[农业科学—水产科学]