薏苡叶乙酸乙酯提取物的体外抗氧化活性研究  

Study on the in Vitro Antioxidant Activity of the Ethyl Acetate Extracts from Coix lachryma-jobi L. Leaves

作　　者：李远辉[1] 郭圣奇 黄挺章 黄莹[1] 卢善善 马忠丽[1] 黄锁义[3] 

机构地区：[1]右江民族医学院临床学院,广西百色533000 [2]右江民族医学院护理学院,广西百色533000 [3]右江民族医学院药学院,广西百色533000

出　　处：《时珍国医国药》2015年第5期1051-1053,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81360684);广西自然科学基金(No.2011GXNSFA018046);广西中医药科技专项资助课题(No.GZKZ 10-128);广西中医药管理局课题(No.gzzc1047);广西医学科学实验中心开放基金专项资助项目(No.KFJJ2011-04)

摘　　要：目的研究薏苡叶乙酸乙酯提取物的体外抗氧化作用,为充分利用薏苡叶提供参考依据。方法以乙酸乙酯为溶剂,制备薏苡叶乙酸乙酯提取液,采用分光光度法对薏苡叶乙酸乙酯提取物清除DPPH自由基和超氧阴离子(·O2-)的能力进行测定,以及测定提取物对金属亚铁离子(Fe2+)的螯合能力,并以丁基羟基茴香醚(BHA)作为对照品与样品的螯合能力进行比较。结果薏苡叶乙酸乙酯提取物具有较强的清除DPPH自由基和超氧阴离子自由基(·O2-)的能力,且具有较强螯合Fe2+的能力。结论薏苡叶乙酸乙酯提取物具有较强的抗氧化能力,其抗氧化性与浓度间存在明显的量效关系,浓度越高抗氧化能力越强。Objective To study in vitro antioxidant activity of the ethyl acetate extracts from Coix lachryma- jobi L. leaves,and provide the theory to make use of Coix lachryma- jobi L. leaves. Methods The ethyl acetate were extracted as the solvent from Coix lachryma-jobi L. leaves. The ethyl acetate extracts from Coix lachryma- jobi L. leaves scavenging DPPH free radical and superoxide radical( O2^-),capacity were used the spectrophotometry to determine,and to determine the content of metal ferrous ion chelating capacity( Fe^2 +),and butylated hydroxyanisole( BHA) as the chelating ability of standard and sample comparison. Results The ethyl acetate extracts of Coix lachryma- jobi L. leaves had strong scavenging DPPH free radical and superoxide radical( ·O2^-) ability,chelating ability with Fe^2 +.Conclusion The ethyl acetate extracts of Coix lachryma- jobi L. leaves has stronger antioxidant capacity,between the antioxidant activity and concentration there is an obvious dose effect relationship,the higher the concentration of antioxidant ability is stronger when the concentration is higher.

关 键 词：薏苡叶 乙酸乙酯提取物 体外 抗氧化 

分 类 号：R284[医药卫生—中药学]