热毒宁注射液中糖类成分的HPLC-ELSD法测定  

Determination of carbohydrate in Reduning Injection by HPLC- ELSD

作　　者：毕宇安[1,2,3] 王雪[1,2] 张伟[1,2] 李家春[1,2] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]南京中医药大学,江苏南京210046

出　　处：《时珍国医国药》2015年第5期1070-1072,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家重点基础研究发展计划("973"计划)(No.2010CB735604)

摘　　要：目的建立热毒宁注射液中糖类成分测定方法。方法采用Waters Carbohydrate(3.9mm×300mm)氨基键合硅胶色谱柱,蒸发光散射检测器,以乙腈-水(83∶17)为流动相,流速为1.2 ml/min。结果同时测定果糖和葡萄糖的含量,色谱峰分离度良好;果糖浓度在0.4056-4.056μg范围内,其浓度对数-峰面积对数呈良好的线性关系(r=0.9992),平均回收率为102.3%,RSD为2.8%;葡萄糖浓度在0.8348-8.348μg范围内,其浓度对数-峰面积对数呈良好的线性关系(r=0.9999),平均回收率为102%,RSD为2.9%。结论该法简便、快速、准确、可靠,可使果糖和葡萄糖呈现良好的分离度,适用于热毒宁注射液中果糖和葡萄糖含量的测定。Objective To establish a method for the determination of fructose and glucose by HPLC- ELSD in Reduning injection. Methods The aminopropyl column was used with acetonitrile: water( 83∶ 17) as mobile phase at a flow rate of 0. 7 m L / min and ELSD detector,the flow rate was 1. 2ml / min. Results The calibration linear curves of fructose and glucose were within concentrations of 0. 4056 - 4. 056 g and 0. 8348 - 8. 348 g. The average recoveries of fructose and glucose were 102. 3% and 102.0%,RSD were 2. 8% and 2. 9% respectively. Conclusion This method is simple,quick and specific,and suitable for the quality control both of the components in Reduning injection.

关 键 词：热毒宁注射液 果糖 葡萄糖 蒸发光散射检测器 

分 类 号：R285.5[医药卫生—中药学]