新疆维吾尔、汉族变应性鼻炎患者HLA-DRB1、HLA-DQB1基因多态性分析  

Analysis of HLA-DRB1,DQB1 polymorphism in Uyghur and Han patients with allergic rhinitis in Xinjiang

作　　者：崔忠涛[1] 范宇琴[2] 胡斌[2] 阳玉萍[2] 张华[2] 

机构地区：[1]哈尔滨医科大学附属第四医院,哈尔滨150001 [2]新疆医科大学第一附属医院耳鼻喉科,乌鲁木齐830054

出　　处：《新疆医科大学学报》2015年第6期669-674,共6页Journal of Xinjiang Medical University

基　　金：国家自然科学基金(30860310)

摘　　要：目的研究新疆地区维吾尔,汉族蒿属花粉变应性鼻炎(Allergic rhinitis,AR)患者HLA-DRB1、HLADQB1等位基因与蒿属过敏易感性的关系。方法采用聚合酶链反应-序列特异性引物技术(PCR-SSP)对新疆地区50名维吾尔族和50名汉族蒿属花粉变应性鼻炎患者及随机选取的本地区的50名维吾尔族和50名汉族健康人(对照组)的HLA-DRB1、HLA-DQB1等位基因进行检测。结果维吾尔族变应性鼻炎组患者的HLA-DRB1*01基因频率(9%)明显高于对照组(1%),差异有统计学意义(P<0.05),HLA-DQB1*05基因频率(19.4%)明显高于对照组(7%);汉族变应性鼻炎组患者的HLA-DRB1*11基因频率(9%)明显高于对照组(2%),差异有统计学意义。HLA-DQB1等位基因频率与对照组差异无统计学意义。结论 HLA-DRB1*01、HLA-DQB1*05可能是维吾尔族AR发病的易感基因,HLA-DRB1*11可能是汉族AR发病的易感基因。Objective To investigate the association between HLA-DRB, DQB1 alleles and artemisia pollen allergic in Han and Uyghur patients of people in Xinjiang. Methods By using PCR-SSP （sequence-specific primer polymerase chain reaction）, we tested HLA-DRB, DQB1 alleles in fifty Uyghur and fifty Han peo- ple with artemisia pollen allergic rhinitis in Xinjiang. The control group included fifty Uyghur and fifty Han people in the region. Results The statistical results showed that the frequencies of HLA-DRB1 ＊ 01 were significantly higher in the Uyghur group with allegic rhinitis（9 %） than in the control group（1 %） （P 〈0.05） and the frequencies of HLA-DQB1 ~ 05 were significantly higher in the Uyghur group with allegic rhinitis（19.4%） than in the control group（7%） （P 〈0.05）. The frequencies of HLA-DRB1 ＊ 11 were higher in the Han group with allergic rhinitis（9%） than in the control group （2%） （P 〈0.05）. The other alleles frequencies had no significant difference in the allergic rhinitis group and in the control group. Con- clusion The investigation indicated that HLA-DRB1 ＊ 01, HLA-DQB1 ＊ 05 might be the inducing factorsin allergic rhinitis in Uyghur patients. HLA-DRB1 ＊ 11 might be associated with allergic rhinitis in Han pa- tients.

关 键 词：变应性鼻炎 基因 HLA 聚合酶链反应 

分 类 号：R765[医药卫生—耳鼻咽喉科]