AtMYB44与ABI1竞争性结合ABA受体RCAR1的研究  

The research of AtMYB44 and ABI1 via competitive binding to ABA receptor RCAR1 in Arabidopsis thaliana

作　　者：赵哲[1] 李德款[1] 袁德志[1] 王晓玉[1] 李旭锋[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2015年第3期663-667,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(31171586);国家转基因重大专项(2011ZX08009-003-002)

摘　　要：为了进一步确定AtMYB44、ABI1、RCAR1三者之间的关系,本文进行了竞争性pull down和ABI1磷酸酶活性的测定实验,结果表明AtMYB44与ABI1能够竞争性结合RCAR1,并且这种竞争性结合依赖于ABA,AtMYB44能够降低RCAR1对ABI1活性的抑制,这为深入研究ABA信号转导途径奠定了基础.In order to determine the relationship among the AtMYB44, ABI1 and RCAR1, competitive GST pull down assay and ABI1 phosphatase activity assay were used in this study, these results sugges- ted that AtMYB44 and ABI1 compete in vitro for binding to RCAR1 in the presence of ABA. In addi- tion, AtMYB44 reduces the inhibitory effect of RCAR1 on ABI1 phosphatase activity in the presence of ABA, which build the foundation for the ABA signal pathway study.

关 键 词：竞争性蛋白沉降技术 RCAR1 ABI1 AtMYB44 

分 类 号：Q945[生物学—植物学]