A New Rapid and Batch-oriented Crushing Method for DNA Extraction from Maize Leaves  

一种快速批量粉碎玉米叶片提取DNA新方法的初步研究（英文）

作　　者：刘鹏飞[1] 陈趣[1] 曾慕衡[1] 王晓明[1] 蒋锋[1] 

机构地区：[1]仲恺农业工程学院作物研究所,广东广州510225

出　　处：《Agricultural Science & Technology》2015年第5期945-946,950,共3页农业科学与技术（英文版）

基　　金：Supported by Special Fund for Agro-scientific Research in the Public Interest of China(201303008);Science and Technology Plan Project of Guangdong Province(2012B020301006);Key Breeding Project for Special Maize of Department of Agriculture of Guangdong Province(B3071328)~~

摘　　要：The crushing of leaves is the most time and effort-consuming part in DNA extraction which is a fundamental step in the study of molecular biology. In this study, a new rapid and batch-oriented crushing method for DNA extraction from maize leaves was developed. In addition, the practicability of the developed method in molecular marker-assisted breeding was verified using SSR molecular maker technology so as to provide a rapid, batch-oriented, low-cost and non-toxic leafcrushing method for a large number of molecular marker tests, improving test efficiency.DNA提取是分子生物学研究的基础步骤,而叶样研磨是提取步骤中最为费时费力的环节。该研究探索出一种快速、批量粉碎玉米叶片以提取DNA的方法,并以SSR分子标记试验验证此方法在分子标记辅助育种工作的应用的可行性,为大容量样品分子标记试验提供一种快速、批量、成本低、无毒安全的粉碎叶样的方法,提高试验效率。

关 键 词：DNA extraction SSR molecular marker MAIZE Rapid and batch-oriented crushing method 

分 类 号：S513[农业科学—作物学]