耳叶牛皮消化学成分的分离与鉴定  

Isolation and identification of chemical constituents from Cynanchum auriculatum

作　　者：李晓鹏[1] 郭娜[1] 许枬[1] 徐晓雪[1] 韩伟健[1] 林晓彤[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《沈阳药科大学学报》2015年第5期347-352,共6页Journal of Shenyang Pharmaceutical University

基　　金："十二五"国家科技支撑计划课题--蒙药产业化共性关键技术研究(2012BAI28B00)

摘　　要：目的深入研究耳叶牛皮消的化学成分,以期更好地开发利用耳叶牛皮消。方法采用硅胶柱色谱、ODS柱色谱和HPLC制备色谱等技术分离纯化耳叶牛皮消的化学成分,经波谱学方法鉴定其化学结构。结果从耳叶牛皮消的根中分离得到11个化合物,即4-羟基苯乙酮(4-hydroxy acetophenone,1),1-(4-羟基苯基)-丙酮(1-(4-hydroxyphenyl)-2-propanone,2),本波苷元(pnupogenin,3),12-O-ikemaoyl-20-dihydro-lineolon(4),凯德苷元(kidjolanin,5),藦萝苷元(metaplexigenin,6),告达亭(cautatin,7),告达亭3-O-β-D-吡喃磁麻糖苷(caudatin 3-O-β-D-cymaropyranoside,8),去乙酰藦萝苷元3-O-β-D-吡喃磁麻糖苷(deacetylmetaplexigenin 3-O-β-D-cymaropyranoside,9),告达亭3-O-β-D-吡喃磁麻糖基-(1→4)-β-D-吡喃洋地黄毒糖苷(caudatin 3-O-β-D-cymaropyranosyl-(1→4)-digitoxopyranoside,10),告达亭3-O-β-D-吡喃夹竹桃糖基-(1→4)-β-D-吡喃磁麻糖苷(caudatin 3-O-β-D-oleandropyranosyl-(1→4)-cymaropyranoside,11)。结论化合物2,3,4,9和10为首次从耳叶牛皮消植物中分离得到。Objective To study the chemical constituents of Cynanchum auriculatum.Methods The compounds were separated and purified by column chromatography.Their structures were confirmed on the NMR spectroscopic analyses.Results Eleven compounds were obtained from the alcohol extract of the C.auriculatum.Their structures were elucidated as 4-hydroxy acetophenone（1）,1-（4-hydroxyphenyl）-2-propanone（2）,pnupogenin（3）,12-O-ikemaoyl-20-dihydro-lineolon（4）,kidjolanin（5）,metaplexigenin（6）,cautatin（7）,caudatin 3-O-β-D-cymaropyranoside（8）,deacetylmetaplexigenin 3-O-β-D-cymaropyranoside（9）,caudatin 3-O-β-D-cymaropyranosyl-（1 → 4）-digitoxopyranoside（10） and caudatin 3-O-β-D-oleandropyranosyl-（1→4）-cymaropyranoside（11）.Conclusions Compounds 2,3,4,9 and 10 are isolated from C.auriculatum for the first time.

关 键 词：耳叶牛皮消 C21甾 本波苷元 告达亭 化学成分 结构鉴定 

分 类 号：R284.2[医药卫生—中药学] R914[医药卫生—中医学]