活泼酯法合成绿原酸人工抗原及其鉴定  

Synthesis of artificial antigen of chlorogenic acid by active ester method and its identification

作　　者：秦美蓉[1,2] 徐静华[1] 刘远平[1,2] 王平[2] 冼静雯[2] 王晓炜[2] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [2]深圳市药品检验所,广东深圳518057

出　　处：《沈阳药科大学学报》2015年第5期386-389,393,共5页Journal of Shenyang Pharmaceutical University

基　　金：中国食品药品检定研究院中青年发展基金项目(2013WA1);深圳药品质量标准研究重点实验室

摘　　要：目的人工合成绿原酸(chlorogenic acid,CGA)完全抗原并进行鉴定,为制备绿原酸单克隆抗体奠定基础。方法采用活泼酯法,将绿原酸分别与牛血清白蛋白(bovine serum albumin,BSA)和卵白蛋白(ovalbumin,OVA)进行偶联,用紫外扫描法、SDS-PAGE电泳法进行鉴定;间接ELISA法测定免疫小鼠血清抗体效价。结果经紫外扫描法和电泳法检测,CGA与载体蛋白成功偶联,免疫小鼠后获得的多抗血清效价达到1×106。结论成功合成绿原酸完全抗原,可用于绿原酸单克隆抗体的制备。Objective To prepare the monoclonal antibody of chlorogenic acid（CGA）,synthesize and identify the artificial complete antigen of CGA.Methods The immunizing antigen（CGA-BSA） and coating antigen（CGA-OVA） were synthesized by active ester method.The characterization of synthesis was identified by UV scanning,SDS-PAGE.The antiserum of immunized mice was tested by indirect ELISA.Results CGA was successfully linked to carrier.Indirect ELISA showed that the antiserum titer of immunized mice were above 1 × 10^6.Conclusions The complete antigen of CGA has been successfully synthesized,which can be used further for preparing monoclonal antibody.

关 键 词：活泼酯法 绿原酸 人工抗原 

分 类 号：R927.1[医药卫生—药学]