犬重度心肌挫伤动物模型的建立及其应用价值  

Animal model construction of dog severe myocardial contusion and its application value

作　　者：张为民[1] 赵兴吉[1] 杜文华[2] 熊秀勤[2] 

机构地区：[1]重庆市急救医疗中心胸心外科,400014 [2]第三军医大学大坪医院野战外科研究所超声诊断科,重庆400042

出　　处：《重庆医学》2015年第15期2037-2039,共3页Chongqing medicine

摘　　要：目的建立犬重度心肌挫伤（MC）的动物模型。方法选用12只犬,体质量（11.36±1.50）kg,采用直接撞击犬左胸前裸区,设置驱动压为800kPa,撞击面积16.61cm^2,胸壁向内压缩4-6cm,建成MC模型。伤后8h行病理检查,根据简明损伤定级标准（AIS,2005更新本）中MC的伤情评定方法进行分级。结果受创区心外膜、心内膜出现散在大面积出血斑,未受创区无明显异常。以左心室横向切开冠状面病理检测,切面8h坏死面积占总面积的（39.78±9.07）%,为重度MC。结论该研究中所建立的犬重度MC模型稳定和可重复性好,能够较准确模拟临时MC模型。Objective To establish an animal model of dog severe myocardial contusion （MC） .Methods 12 dogs with the body weight of （11 .36 ± 1 .50）kg were selected .The BIM‐Ⅱ type biological impact machine was adopted to directly impact the bare area of the left chest by the driving pressure of 800 kPa ,the impact area was 16 .61 cm^2 and the chest wall was inward compressed within 4-6 cm ,the severe myocardial contusion model was established .The pathological examination was taken at 8 h after injury and grading was performed according to the Abbreviated Injury Scale （AIS ,update in 2005） .Results The scattered large area bleeding spots appeared in the epicardium and endocardium of wound area .No obvious abnormality was found in the non - wound area .The pathological examination was performed based on the coronal plane of the left ventricular cross section .The percentage of the section 8 h necrosis area to the total area was （39 .78 ± 9 .07）% .Conclusion The established dog severe myocardial contusion model is stable and reproducible ,which can accurately simulate the temporary MC mode .

关 键 词：心肌挫伤 出血 撞击伤 

分 类 号：R542.2[医药卫生—心血管疾病] R-332[医药卫生—内科学]