吖啶红荧光猝灭法测定水样及果皮中的痕量铜  

Fluorimetric Quenching Method Determination of Trace Copper(Ⅱ)in Water and Fruit Peel with Acridine Red

作　　者：苏布道[1] 余静 覃松[1] 

机构地区：[1]内江师范学院果蔬类废弃物资源化四川省高等学校重点实验室,四川内江641112 [2]仁寿县新科中等职业技术学院,四川眉山620500

出　　处：《广州化工》2015年第10期115-117,共3页GuangZhou Chemical Industry

基　　金：果蔬类废弃物资源化四川省高等学校重点实验室资助;内江师范学院青年基金课题(13ZB04)

摘　　要：磷酸盐缓冲介质中,痕量Cu（Ⅱ）对过氧化氢氧化吖啶红的反应具有催化作用,导致吖啶红在550nm处的荧光强度发生明显的猝灭,以此建立了测定痕量铜的新方法。在最优化条件下,方法的线性范围为8.0×10^-4~9.2×10^-3μg·mL^-1,检出限为7.3×10^-4μg·mL^-1,用于水样及果皮样品中痕量Cu（Ⅱ）的测定,回收率在97.5%~103.5%之间。在40℃条件下,猝灭反应的活化能Ea=87kJ·mol^-1,表观速率常数k=1.05×10^-3s^-1,且Cu（Ⅱ）对体系的荧光猝灭属动态猝灭。In a phosphate buffer solution, trace copper（ Ⅱ） had a catalytic effect on oxidation reaction of acridine red by hydrogen peroxide. The fluorescence intensity of acridine red decreased with the increase of the concentration of copper（ Ⅱ）. A new method was established for determination of trace copper（ Ⅱ）. The linear range for the determination was between 8.0×10^-4 and 9.2×10^-3μg·mL^-1 , and the detection limit was 7.3×10^-4μg·mL^-1. The proposed method was applied to the determination of trace copper in practical samples with satisfactory results and recovery rate was 97. 5% ~ 103.5%. The apparent activation energy of the catalytic reaction was 87 kJ · mol^-1, and the apparent rate was 1.05× 10^-3 s^-1 under the condition of 40 ℃. The quenching mechanism was determined as dynamic quenching.

关 键 词：铜 吖啶红 催化动力学 荧光猝灭 

分 类 号：O652.3[理学—分析化学]