苦瓜总皂苷对2型糖尿病大鼠胰岛素信号转导通路的影响  

Effect of Total Saponins from Fructus Momordicae Charantiae on Insulin Signal Transduction Pathway in Type 2 Diabetes Mellitus Rats

作　　者：马春宇[1] 王慧娇[1] 于洪宇[2] 耿丽晶[3] 

机构地区：[1]辽宁医学院实验诊断学教研室,辽宁锦州121000 [2]辽宁医学院护理学院,辽宁锦州121000 [3]辽宁医学院食品学院,辽宁锦州121000

出　　处：《中药新药与临床药理》2015年第3期289-294,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：辽宁省自然科学基金项目(项目编号:2014022043)

摘　　要：目的探讨苦瓜总皂苷对2型糖尿病大鼠胰岛素信号转导通路的调节作用并揭示其改善胰岛素抵抗作用的机制。方法用高脂高糖饮食喂养结合小剂量链脲佐菌素(STZ)注射复制2型糖尿病大鼠模型,分别以苦瓜总皂苷低、中、高剂量(100,200,400 mg·kg-1·d-1)和二甲双胍(50 mg·kg-1·d-1)灌胃治疗,共8周。取肝脏组织分别用RT-PCR方法和Western blot方法检测蛋白酪氨酸磷酸酶1B(PTP-1B)、细胞因子信号抑制物3(SOCS-3)和c-Jun氨基末端激酶(JNK)的mRNA和蛋白表达量。结果与模型组比较,苦瓜总皂苷各剂量组SOCS-3和JNK的mRNA和蛋白表达量均降低,PTP-1B m RNA也降低(P<0.01)。而且SOCS-3和JNK的mRNA和蛋白表达量随着苦瓜总皂苷剂量的增加表达量逐渐递减,呈剂量依赖关系。结论苦瓜总皂苷改善胰岛素抵抗作用机制很可能是下调SOCS-3、JNK mRNA和蛋白的表达量进而使胰岛素信号转导通路的作用增强。Objective To explore the mechanism of Fructus Momordicae Charantiae total saponins（MCTS） in ameliorating insulin resistance through investigating the influence of MCTS on insulin signal transduction pathway in type 2 diabetes mellitus（T2DM）rats. Methods T2 DM rat model was established by feeding with high lipid and sugar diets combined with injection of low-dose streptozotocin. The model rats were administrated with MCTS at100,200,400 mg·kg^-1 and metformin at 50 mg·kg^-1for 8 weeks. At the end of the experiment,liver tissues were sampled for the detection of protein tyrosine phosphatase 1B（PTP-1B）,suppressors of cytokine signaling 3（SOCS-3）and c-Jun N-terminal kinase（JNK）m RNA and protein by RT-PCR and western blotting method. Results The m RNA and protein expression levels of SOCS-3 and JNK were decreased obviously in MCTS groups compared to the T2 DM model group,and the decrease became obvious with the increase of the dosage of MCTS. PTP-1B only showed lower m RNA expression level in MCTS groups than that of the T2 DM model group. The m RNA and protein expression amount of SOCS-3 and JNK decreased with the increasing dosage of MCTS. Conclusion The mechanism of MCTS in ameliorating insulin resistance may be related with the increase of the insulin signal transduction pathway sensitivity through down-regulating the m RNA and protein expression of SOCS-3 and JNK.

关 键 词：苦瓜总皂苷 2型糖尿病 胰岛素抵抗 SOCS-3 JNK 

分 类 号：R285.5[医药卫生—中药学]