HPLC法测定补骨脂微囊中补骨脂素及异补骨脂素的含量  

Determination of Psoralen and Isopsoralen in Fructus Psoraleae Microcapsules by HPLC

作　　者：黄辉庆[1] 袁小红[1,2] 周健韬[3] 胡学军[1] 

机构地区：[1]广东省中医院,广东广州510120 [2]广东省中医药科学院,广东广州510006 [3]广州中医药大学,广东广州510006

出　　处：《中药新药与临床药理》2015年第3期377-380,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省中医药管理局课题(2008125);广东省科技厅课题(0903124)

摘　　要：目的建立补骨脂微囊中补骨脂素和异补骨脂素的含量测定方法。方法采用高效液相色谱法,以Diamonsil（Ⅱ）-C18柱（4.6 mm×250 mm,5μm）为固定相,以甲醇：水（52∶48）为流动相,流速为1 m L·min-1,测定波长为245 nm。结果补骨脂微囊中补骨脂素含量在0.058~0.29μg之间,异补骨脂素含量在0.047~0.235μg之间线性关系良好。补骨脂素线性回归方程为：Y=211373X-649200,r=0.9998;异补骨脂素为Y=152289X-167131,r=0.9996。补骨脂微囊中补骨脂素和异补骨脂素的总含量均值为16.8 mg·g-1。结论该测定方法稳定、简便、重复性好,可以有效控制补骨脂微囊的质量。Objective To establish a method for the simultaneous determination of psoralen and isopsoralen in Fructus Psoraleae Microcapsules. Methods HPLC was carried out on the column of Diamonsil（II）-C18column（4.6 mm×250mm,5 μm）. The mobile phase consisted of methanol-water（52∶48）,the flow rate was 1.0 m L·min-1and the UV detection wavelength was 245 nm. Results Good linearity of psoralen was showed within the range of 0.058 ~ 0.290 μg and that of isopsoralen within the range of 0.047~0.235 μg. The equation of linear regression for psoralen was Y=211373X-649200, r=0.9998, and for isopsoralen was Y=152289X-167131, r=0.9996. The total content of psoralen and isopsoralen in the microcapsules was 16.8 mg·g-1. Conclusion The method is stable, accurate and simple,and is suitable for the quality control of Fructus Psoraleae Microcapsules.

关 键 词：补骨脂微囊 补骨脂素 异补骨脂素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]