金纳米颗粒的自组装修饰及其生物学效应  

Biological Effects of Gold Nanoparticles Modified by Self-Assembled Monolayers

作　　者：周添[1] 董庆磊[1] 沈阳[2] 葛淑萍[1] 张勤[1] 罗祥林 王贵学[1] 

机构地区：[1]重庆大学重庆市血管植入物工程实验室,重庆400044 [2]四川大学,四川成都610041

出　　处：《稀有金属材料与工程》2015年第5期1203-1208,共6页Rare Metal Materials and Engineering

基　　金：国家科技支撑计划(2012BAI18B02);国家自然科学基金(11332003;31370949);中央高校基本科研业务费(CQDXWL-2012-120)

摘　　要：为了探究金纳米颗粒(Au NP)s的表面自组装亲疏水性修饰及其生物学效应,通过自组装技术制备SH-(CH2)11OH和SH-(CH2)11CH3不同配比修饰的Au NPs,并用透射电子显微镜(TEM)、纳米粒度仪和傅里叶变换红外光谱(FT-IR)检测修饰后的表面形貌与结构特征。用体视显微镜观察Au NPs修饰后对斑马鱼胚胎/幼鱼表型的影响。用MMT、NO含量试剂盒、总抗氧化能力(T-AOC)试剂盒检测Au NPs修饰后对人脐静脉内皮细胞(HUVECs)毒性和功能指标的影响。利用电感耦合等离子体发射光谱和TEM检测Au NPs修饰后被HUVECs摄入情况。结果表明,用SH-(CH2)11OH修饰比用SH-(CH2)11CH3修饰的Au NPs毒性大,当修饰Au NPs的SH-(CH2)11OH和SH-(CH2)11CH3配比为50:50时毒性最小。The gold nanoparticles（Au NPs） were modified by self-assembled minelayers（SAMs） that were mixed with different ratios of SH-（CH2）11CH3 to SH-（CH2）11OH. The surface morphologies and structures of unmodified and modified Au NPs were characterized by transmission electron microscopy（TEM）, nanoparticle size analyzer, and Fourier transform infrared spectrometer（FT-IR）; then zebrafish and human umbilical vein endothelial cells（HUVECs） were used to assess the SAM-Au NPs toxicity; TEM and inductively coupled plasma atomic emission spectroscopy（ICP-AES） were used to assess the cellular uptake of SAMs-modified Au NPs（SAM-Au NPs）.Results reveal that Au NPs are modified successfully with SAMs. SH-（CH2）11OH-modified Au NPs cause more serious zebrafish malformations. SAM-Au NPs could improve the nitrogen monoxide（NO） content and total antioxidation capacity（T-AOC） of HUVECs.And when the ratio of SH-（CH2）11CH3 to SH-（CH2）11OH is 50：50, the T-AOC is strongest. MTT assay show that SAM-Au NPs lower the cytotoxicity at that ratio, but when the ratio is 0：100 or 100：0, the cytotoxicity of modified Au NPs is higher. In addition, the amount of cellular uptake of SH-（CH2）11OH-modified Au NPs is higher as compared with SH-（CH2）11CH3-modified Au NPs.

关 键 词：金纳米颗粒 自组装单分子层 亲/疏水性基团 生物学效应 斑马鱼 

分 类 号：TB383.1[一般工业技术—材料科学与工程] O614.123[理学—无机化学]