大花蕙兰快速繁殖体系的初步建立  

Preliminary Establishment of Rapid Propagation System of Cymbidium Hybridum

作　　者：胡燕梅[1] 夏景波[2] 方中明[3] 郭云贵[3] 杨永会[3] 

机构地区：[1]江汉大学期刊社,湖北武汉430056 [2]暨南大学生命科学技术学院,广东广州510632 [3]武汉生物工程学院生物科学与技术学院,湖北武汉430415

出　　处：《江汉大学学报（自然科学版）》2015年第3期248-252,共5页Journal of Jianghan University：Natural Science Edition

基　　金：国家自然科学基金青年基金项目(31301250);湖北省教育厅科学技术研究项目(B20094008);武汉市高校科学研究项目(2008K079)

摘　　要：探讨了4种大花蕙兰外植体诱导原球茎形成的效果和不同激素配比对茎尖原球茎诱导及增殖与分化培养的影响。结果表明:诱导原球茎适宜外植体为茎尖,培养60 d后诱导率达到76.0%。而其他3种外植体均不能有效诱导原球茎形成;其中0.5 mg/L 6-BA+0.1 mg/L NAA激素组合诱导茎尖原球茎形成率达到87.8%;而茎尖原球茎在各激素组合处理中增殖率均能达到100%。原球茎在1.5 mg/L KT+0.5 mg/L IBA激素组合中增殖与分化效果最好,增殖系数和分化系数分别达到5.19和2.80。The formation effects of protocorm of cymbidium induced by four kinds of explants are dis?cussed ,as well as the influence of hormone proportion to proliferation and differentiation of protocorm in?duction. The experiment results showed the best explants for protocorm induction were stem tips ,the in?ducing rate reached 76. 0%after 60 days culture. The three other explants could not effectively induce the protocorm. With 0. 5 mg/L 6-BA+0. 1 mg/L NAA treatment,the formation rate of protocorm induced by stem tip reached 87. 8%,and the proliferation rate of protocorm induced by stem tip with each hormone treatment all reached 100%,the proliferation and differentiation of protocorm were the best with the treat?ment of 1. 5 mg/L KT+0. 5 mg/L IBA ,the proliferation coefficient and the differentiation coefficient are 5. 19 and 2. 80 ,respectively.

关 键 词：大花蕙兰 快速繁殖 茎尖 原球茎 增殖与分化 

分 类 号：S682.31[农业科学—观赏园艺]