甘肃红豆草无色花青素还原酶LAR基因的克隆和表达分析  

Cloning and Expression Analysis of a Leucoanthocyanidin Reductase( LAR) Gene from Onobrychis viciifolia cv. Gansu

作　　者：陈春艳[1,2,3,4] 马晖玲[1,3,4] 董文科[1,3,4] 

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070 [2]河南科技大学农学院,河南洛阳471003 [3]草业生态系统教育部重点实验室,甘肃兰州730070 [4]中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2015年第6期177-187,共11页Acta Prataculturae Sinica

基　　金：甘肃省科技厅科技支撑计划项目(1104NKCA087);国家自然科学基金项目(31200299)资助

摘　　要：以甘肃红豆草为材料,采用RT-PCR法克隆无色花青素还原酶LAR基因,分析该功能基因在甘肃红豆草不同器官表达的差异性。结果表明,克隆的甘肃红豆草LAR基因,与Gen Bank已报道LAR基因(登录号:HM152981)序列相似性达到98.34%,其ORF长为1089 bp,编码362个氨基酸残基;其编码的氨基酸序列与不同属豆科物种的相似性存在较大的差异。基因序列已注册到Gen Bank,序列登录号为KP013623。LAR基因在甘肃红豆草不同器官表达差异较大;其中叶中表达量最高,其次是花和果,茎中表达量最低,这与器官间缩合单宁含量的变化规律一致。推测其表达与甘肃红豆草缩合单宁器官间的积累差异有关。这些研究结果也为探索缩合单宁积累和表达调控的机制提供基础。A leucoanthocyanidin reductase (LAR)gene was cloned using RT-PCR from Onobrychis viciifolia (sainfoin)cv.Gansu,and the level of LAR gene expression was analyzed in different plant organs.The LAR gene sequence from O.viciifolia cv.Gansu shared a high level of similarity (98.34% homology)with an O. viciifolia LAR gene in GenBank (accession No:HM152980).However,the coding sequence of amino acids is obviously different from those in other genera of the Fabaceae.The open reading frame (ORF)comprised 1089 bp,encoding 362 amino acid residues.The sequence of the LAR gene from the present study was submitted to GenBank with the accession number KP013623.LAR expression differed between plant organs of O.viciifolia cv.Gansu.LAR expression was highest in leaves,followed by flowers and fruit,and was lowest in stem tis-sues,which corresponded to concentration of condensed tannins in different organs of O.viciifolia cv.Gansu. It is inferred that LAR gene expression is in some way involved in condensed tannin accumulation in O.viciifoliacv.Gansu.These results provide fundamental information and a method for exploring the regulation mecha-nism of condensed tannin accumulation.

关 键 词：甘肃红豆草 RT-PCR 无色花青素还原酶 克隆与表达 

分 类 号：S541.4[农业科学—作物学]