西瓜花叶病毒三抗体夹心法与纸质免疫检测传感器检测方法的建立  

TAS-ELISA and Electrochemical Paper Assisted Immunosensor for the Detection of Watermelon Mosaic Virus

作　　者：孔德昭[1] 朱怡橙[2] 马伟[1] 徐丽广[1] 胥传来[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]淮阴工学院生命科学与化学工程学院,江苏淮安223003

出　　处：《食品与生物技术学报》2015年第4期361-365,共5页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2012BAC01B07)

摘　　要：利用西瓜花叶病毒2号外壳蛋白的小鼠单克隆抗体与兔多克隆抗体,建立两种针对西瓜花叶病毒2号的检测方法。运用现有的抗体建立了三抗体夹心酶联免疫吸附法(TAS-ELISA);运用碳纳米管与抗体包裹滤纸制备纸质免疫检测传感器,再基于电化学工作站建立电化学纸质免疫检测传感器检测方法。两种方法对实际样品检测限分别为0.15、0.20μg/m L,检测时间分别为3 h和30 min。根据结果分析比较,三抗体夹心法具有较高的检测灵敏度,而电化学纸质免疫传感器检测方法具有更短的检测时间。Using a mice monoclonal antibody and a rabbit polyclonal antibody of the watermelonmosaic virus(WMV)- 2 coat protein, we developed two detection methods for WMV- 2. The trimerantibody sandwich enzyme-linked immunosorbent assay(TAS-ELISA) method was established basedon two different antibodies; the electrochemical paper assisted immunosensor detection method wasestablished by combining the electrochemical workstation with the paper assisted immunosensorprepared by the carbon nanotube and the antibody-coated filter paper. The detection limit was 0.15 and0.2 μg/ml, and the testing time was 3 hours and 30 minutes for two methods, respectively. According tothese results, the TAS-ELISA method had a higher detection sensitivity, and the electrochemical paperassisted immunosensor detection method had a shorter testing time.

关 键 词：西瓜花叶病毒2号 外壳蛋白 三抗体夹心法 电化学纸质免疫检测传感器 

分 类 号：S432.41[农业科学—植物病理学]