甘油浓度对红细胞冻干保存效果的影响  

Influence of glycerin on lyophilized preservation of red blood cells

作　　者：陈奕冰[1] 张莹[1] 田力 宋宁[1] 张嵘[1] 陈强[1] 刘忠[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2015年第4期370-373,F0001,共5页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金面上项目(81370669)

摘　　要：目的探讨甘油浓度浓度对红细胞冷冻干燥保存的回收率、溶血率及残余水含量的关系。方法以甘油浓度分别为3%、6%、9%、12%、15%、18%、21%(w/v)的冻干保护剂处理红细胞,冻干,用显微镜观察细胞形态、血细胞计数仪检测细胞数、分光光度计测定游离血红蛋白量,分析细胞复水后红细胞计数、溶血情况。用热重法测定冻干红细胞水分含量,分析不同保护剂组红细胞的水分含量变化情况。结果复水后红细胞形态正常,甘油浓度为9%、12%、15%时,红细胞回收率分别为(77.08±9.41)%、(84.37±3.42)%、(80.21±9.20)%,红细胞溶血率分别为(19.82±2.23)%、(17.66±1.17)%、(15.86±2.23)%,相应的冻干红细胞残余水分含量为(19.43±1.36)%、(22.89±1.57)%、(26.17±1.09)%。结论保护剂中甘油浓度影响红细胞冻干保存的效果;甘油浓度为(9-15)%时对冻干保存的红细胞损伤较小;冻干红细胞残余水分含量随保护剂中甘油浓度的增高而增高。Objective To investigate the effect of different concentrations of glycerol on the process of rehydration of RBCs and the proportion of residual water in order to provide basic data for further study. Methods RBCs were subjected to different concentrations of glycerol(3%,6%,9%,6%,15%,18%,15%)( w / v),and then underwent freeze-drying by freeze dryer. Associate parameters,such as cell morphology andnumber of blood cells,were measured by automatic blood cell counter,while free hemoglobin in supernatant was measured by spectrophotometer. The results were statistically analyzed. The proportion of residual moisture was measured by thermogravimetric analyzer and the change of water content was analyzed in different groups. Results Under light microscope,the cell morphology was normal after rehydration. When the concentration of glycerol was 9%-15%,the recovery of RBCs reached(77. 08 ± 9. 41) %-(80. 21 ± 9. 20) %,the hemolysis rate was(19. 82 ± 2. 23) %-(15. 86 ± 2. 23) %,and the content of residual water was(19. 433 ± 1. 355) %-(26. 173 ± 1. 087)%. Conclusion When the concentration of glycerol reaches between 9% and 15%,the damage on RBCs is the least. The proportion of residual water increases as the concentration of protective regents increases.

关 键 词：红细胞 冻干保存 甘油浓度 残余水含量 红细胞形态 红细胞回收率 红细胞溶血率 

分 类 号：R331.141[医药卫生—人体生理学] O623.413[医药卫生—基础医学]